白鸡屎藤挥发油抗肠炎沙门菌内毒素作用的分析

为了探讨白鸡屎藤挥发油抗肠炎沙门菌内毒素的作用,将420只7日龄三黄鸡随机分成A、B、C、D、E、F组并对A、B、C组鸡按100μg/mL(1mL)预防用药,在12日龄时对A、B、D、E组口服肠炎沙门菌攻毒,攻毒24h后对A、D组按100μg/mL白鸡屎藤挥发油1mL进行滴鼻给药治疗,同时将不同质量浓度的白鸡屎藤挥发油与内毒素混合对另外90只雏鸡进行肌肉注射攻毒,并进行试验鸡与攻毒鸡临床死亡情况统计、病理组织学观察和肝与血液生化指标测定。结果显示,白鸡屎藤挥发油对肠炎沙门菌内毒素具有灭活作用,可缓解血清ALT和AST活性的升高或使其降低(P<0.01或P<0.05),提升肝及血清中SOD的活性(P<0.01或P<0.05),使肝中MDA含量下降和延缓其升高。结果表明,白鸡屎藤挥发油具有抗肠炎沙门菌内毒素的作用,其机理与其保肝作用和抗氧化损伤作用有关。

Role of volatile oil of white Paederia scandens in endotoxin of Salmonella Enteritidis

To study the effect of volatile oil of white Paederia scandens on the endotoxin of Salmonella Enteritidis,420 7-day-old San-Huang broilers were randomly divided into six groups(A,B,C,D,E and F group),and the broilers in Groups A,B,C were offered preventive medication intranasally at 1 mL(100 μg/mL).At 12-day-old,Groups A,B,C,D were treated orally with S.Enteritidis for modeling,and Groups A and D were treated intranasally with volatile oil at 1 mL(100 μg/mL) after 24 h.And 90 7-day-old San-Huang broilers were conteracted toxic substances which were mixed by different concentrations of the volatile oil and endotoxin.Meanwhile,the changes of ALT,AST,SOD and MDA in serum and the liver of the broilers were tested.The results showed that the endotoxin of S.Enteritidis could be inactivated by the vo-latile oil of white P.scandens,with the activities of ALT and AST kept being increased later or decreased(P<0.01 or P<0.05),the SOD activities in the liver and serum being promoted(P<0.01 or P<0.05),and the content of MDA kept being decreased or increased later under the condition of administration with nasal drops.The conclusion was that the endotoxin of S.Enteritidis could be inactivated by the volatile oil of white P.scandens,which was concerned with hepatoprotective effect and anti-oxidant.