| 胶体金标记检测大麻单克隆抗体免疫试剂盒研制 |
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| 引用本文： | 陈连康,曾立波,赵志强,陈安,丛林. 胶体金标记检测大麻单克隆抗体免疫试剂盒研制[J]. 中国法医学杂志, 2002, 17(2): 93-95 |
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| 作者姓名： | 陈连康 曾立波 赵志强 陈安 丛林 |
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| 作者单位： | 1. 上海公安局刑事科学技术研究所,上海,200083
2. 上海美康生物工程有限公司,上海,201315 |
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| 摘 要： | 目的 建立准确、快速、简便的检测尿液中大麻的胶体金免疫层析技术(ICT)。方法 采用柠檬酸三钠还原法制备胶体金颗粒,标记抗主要代谢物四氢大麻酚-9-羧酸,THC与GC/MS检测方法相比,用ICT法的216份尿样,其检测限为50 ng/ml,灵敏度为96.67％,准确性为98.61％。结论 ICT法检测尿液中THC,其特异性强,对确定大麻的存在具有广泛的应用价值。
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| 关 键 词： | 胶体金 单克隆抗体 大麻 四氢大麻酚-9-羧酸(THC) |
| 文章编号： | 1001-5728(2002)02-0093-03 |
| 修稿时间： | 2002-12-01 |
Preparation of the immnno-kit of anti-THC monoclonal antibody labelled with colloidal gold |
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| CHENLiankang,ZENG Libo,ZHAO Zhiqiang,et al.. Preparation of the immnno-kit of anti-THC monoclonal antibody labelled with colloidal gold[J]. Chinese Journal of Forensic Medicine, 2002, 17(2): 93-95 |
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| Authors: | CHENLiankang ZENG Libo ZHAO Zhiqiang et al. |
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| Affiliation: | CHENLiankang,ZENG Libo,ZHAO Zhiqiang,et al./ Shanghai Forensic Science and Technology Research Insititute of the Public Security Bureau,Shanghai 200083 |
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| Abstract: | Objective To develop an accurate, rapid and simple colloidal gold immunochromatographic test (ICT) for detection of THC in urine. Methods Colloidal gold was prepared by reduction of HAuCl4 with trisodium citrate. Anti-THC monoclonal antibody was then labelled with the colloidal gold. THC-BTG conjugate was coated on nitrocellulose membrane to prepare test strip. Result could be detected by naked eyes because the competition for limited antibody binding sites on either THC-BTG conjugate or free THC which may be present in urine samples. Results A total of 216 urine samples were detected for THC by ICT and GC/MS. The detecting limit of the ICT was 50ng/ml, the sensitivity waw 96.67% and the accuracy was 98.61%. Conclusion ICT is a specific assay for detection of THC in urine. |
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| Keywords: | Colloidal gold Anti-IHC monoclonal antibody ll-nor-8-tetrahydrocannabinol-9-car-boxylic acid (THC) |
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