| 少孢节丛孢菌胞外蛋白酶的诱导与生化性质 |
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| 引用本文： | 王瑞,杨莲茹,杨晓野,马亚囡,赵治国,赵晓慧,李文生. 少孢节丛孢菌胞外蛋白酶的诱导与生化性质[J]. 中国兽医科学, 2010, 40(5) |
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| 作者姓名： | 王瑞 杨莲茹 杨晓野 马亚囡 赵治国 赵晓慧 李文生 |
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| 作者单位： | 王瑞,杨莲茹,杨晓野,赵治国,赵晓慧,李文生,WANG Rui,YANG Lian-ru,YANG Xiao-ye,ZHAO Zhi-guo,ZHAO Xiao-hui,LI Wen-sheng(内蒙古农业大学,兽医学院,内蒙古,呼和浩特,010018);马亚囡,MA Ya-nan(内蒙古神元生物工程股份有限公司,内蒙古,呼和浩特,010010) |
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| 基金项目： | 国家自然科学基金，内蒙古自然科学基金 |
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| 摘 要： | 应用不同诱导剂和不同营养组成的液体培养基诱导培养捕食线虫性真菌——少孢节丛孢菌产生发酵液,经浓缩处理后,对发酵液中的蛋白质含量、酶活性以及相关的生化性质进行了测定。结果表明,培养基的组成成分和相关诱导剂能够显著影响少孢节丛孢菌的代谢过程;而且秀丽新杆线虫刺激少孢节丛孢菌产生的培养基滤液和氨基酸刺激所产生的发酵液内含成分不同;蛋白酶活性越高的滤液中,磷酸酶活性越低;产生胞外蛋白酶的最佳培养时间为6d。
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| 关 键 词： | 捕食线虫性真菌 少孢节丛孢菌 胞外蛋白酶 磷酸酶 |
Induction and biochemical characteristics of extracellular protease from Arthrobotrys oligospora |
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| WANG Rui,YANG Lian-ru,YANG Xiao-ye,MA Ya-nan,ZHAO Zhi-guo,ZHAO Xiao-hui,LI Wen-sheng. Induction and biochemical characteristics of extracellular protease from Arthrobotrys oligospora[J]. Chinese Veterinary Science, 2010, 40(5) |
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| Authors: | WANG Rui YANG Lian-ru YANG Xiao-ye MA Ya-nan ZHAO Zhi-guo ZHAO Xiao-hui LI Wen-sheng |
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| Affiliation: | WANG Rui1,YANG Lian-ru1,YANG Xiao-ye1,MA Ya-nan2,ZHAO Zhi-guo1,ZHAO Xiao-hui1,LI Wen-sheng1 (1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China,2.Shen Yuan Bio-engineering Co.,Ltd.,Hohhot 010010,China) |
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| Abstract: | Different liquid medium and inductors were used to produce fermentation broth of the nematode-trapping fungus Arthrobotrys oligospora.The protein concentration,enzyme activity and related biochemical properties of the concentrated broth were determined.Results showed the method could significantly influence metabolic process of A.oligospora,and the composition of the broth filtrate induced by Caenorhabditis elegans was different from that by amino acid.Besides,the broth filtrate with high protease activity ... |
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| Keywords: | nematode-trapping fungi Arthrobotrys oligospora extracellular protease phosphatase |
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