| 伪狂犬病病毒Ea株糖蛋白基因gL的克隆和序列分析 |
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| 引用本文： | 刘正飞,陈焕春,琚春梅,吴斌,何启盖.伪狂犬病病毒Ea株糖蛋白基因gL的克隆和序列分析[J].中国兽医科学,2002,32(11):5-7. |
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| 作者姓名： | 刘正飞 陈焕春 琚春梅 吴斌 何启盖 |
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| 作者单位： | 华中农业大学畜牧兽医学院,湖北,武汉,430070 |
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| 基金项目： | 国家自然科学基金资助项目 (39970 559) |
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| 摘 要： | 根据已发表的基因序列设计了 1对PCR引物 ,以伪狂犬病病毒Ea株 (pseudorabiesvirusEa ,PRVEa)基因组DNA为模板 ,扩增出一大小为 5 11bp的片段。通过酶切和测序证实 ,该基因为伪狂犬病病毒Ea株的又一糖蛋白基因 ,即gL基因。Blast软件分析表明 ,该基因与Kaplan株核苷酸序列的同源性为 94% ,氨基酸序列的同源性为 95 %。将测序结果输入GenBank ,获得登录号AF44 845 6。
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| 关 键 词： | 伪狂犬病病毒Ea株 gL基因 克隆 序列分析 |
| 文章编号： | 1000-6419(2002)11-0005-03 |
| 修稿时间： | 2002年5月8日 |
Cloning and sequence analysis of the glycoprotein gene gL of pseudorabies virus strain Ea |
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| LIU Zheng fei,CHEN Huan chun,JU Chun mei,WU Bin,HE Qi gai.Cloning and sequence analysis of the glycoprotein gene gL of pseudorabies virus strain Ea[J].Veterinary Science in China,2002,32(11):5-7. |
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| Authors: | LIU Zheng fei CHEN Huan chun JU Chun mei WU Bin HE Qi gai |
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| Abstract: | A 511 bp gene segment was amplified by PCR with the template of pseudorabies virus Ea strain genome DNA and a pair of primers designed acording to the published gene sequence. Restriction nuclease enzyme digestion and sequence analysis indicated that the gene segment is gL gene . There are several mutations, which results in the 94% DNA identifity to PRV strain Kaplan. Further analysis demonstrated in PRV Ea gL gene there is 95% amino acid identifity. The gL gene sequence was submitted to GenBank, and the accession number is AF448456. |
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| Keywords: | pseudorabies virus strain Ea cloning sequence analysis |
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