| 红细胞酸性磷酸酶(EAP)型的分布及血痕EAP的检出 |
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| 引用本文： | 卢笛,贾静涛,丁梅. 红细胞酸性磷酸酶(EAP)型的分布及血痕EAP的检出[J]. 中国法医学杂志, 1987, 0(2) |
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| 作者姓名： | 卢笛 贾静涛 丁梅 |
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| 作者单位： | 上海医科大学法医教研室，中国医科大学法医血清学教研室，中国医科大学法医血清学教研室 进修生 |
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| 摘 要： | 本文应用琼脂糖凝胶电泳法对辽宁地区213例汉族随机献血员的红细胞酸性磷酸酶(EAP)型进行了检测,其基因频率为EAP~(?)=0.169,EAP~b-0.831。结合文献资料分析了EAP型分布的种族差异,指出了各人种EAP型分布的特点。用琼脂糖凝胶电泳法,对红细胞溶血液EAP型的最小检出量为2μL(2×3mm滤纸条)及0.5μL(直接加样);对血痕EAP型的最小检出量为7.5μL血液制成的检样。本法在4℃保存4周以内的红细胞溶血液和室温保存25天的血痕均能正确判定EAP的型别。
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| 关 键 词： | 法医血清学 红细胞酸性磷酸酶 基因频率 血痕 |
ON THE DISTRIBUTION OF ERYTHROCYTE ACID PHOSPHATASE(EAP)PHENOTYPES AND THE DETECTION OF EAP FROM BLOODSTAINS |
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| Lu Di,Jia Jingtao,Ding Mei Department ot Forensic Serology Faculty of Forensic Medicine,China Medical University,Shenyang. ON THE DISTRIBUTION OF ERYTHROCYTE ACID PHOSPHATASE(EAP)PHENOTYPES AND THE DETECTION OF EAP FROM BLOODSTAINS[J]. Chinese Journal of Forensic Medicine, 1987, 0(2) |
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| Authors: | Lu Di Jia Jingtao Ding Mei Department ot Forensic Serology Faculty of Forensic Medicine China Medical University Shenyang |
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| Abstract: | The phenotyping of erythrocyte acid phosphatase of blood from 213 chin- ese random donors,Han nationalities in Liaoning China,was performed by the agarose gel electrophoresis.Gene frequencies were:EAP~a=0.169, EAP~b=0.831.The race differences in EAP gene frequecies were compared. The minimal amount meets the requirement for detection of EAP were 2μL o hemolysate and 7.5μL of bloodstains.It could be phenotyping correctly that the hemolysate kept in 4℃ for 4 weeks and the bloodstains kept in 15℃ for 25 days. |
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| Keywords: | Forensic serology EAP phenotypes Gene frequency Bloodstains |
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