| 串联型黄色荧光蛋白表达载体的构建及其在柔嫩艾美球虫中的瞬时表达 |
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| 引用本文： | 李继东,郝力力,刘贤勇,周晓艳,索勋. 串联型黄色荧光蛋白表达载体的构建及其在柔嫩艾美球虫中的瞬时表达[J]. 中国兽医科学, 2010, 40(1) |
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| 作者姓名： | 李继东 郝力力 刘贤勇 周晓艳 索勋 |
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| 作者单位： | 李继东(宁夏大学农学院,宁夏,银川,750021;中国农业大学动物医学院,北京,100193);郝力力(中国农业大学动物医学院,北京,100193;中国医学科学院病原生物学研究所,北京,100730);刘贤勇,周晓艳,索勋(中国农业大学动物医学院,北京,100193) |
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| 基金项目： | 围家自然科学基会项目 |
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| 摘 要： | 为了探索黄色荧光蛋白(YFP)在柔嫩艾美球虫子孢子中的瞬时表达情况,以柔嫩艾美球虫的组蛋白4(Histone4,H4)上游启动序列和肌动蛋白(Actin)下游调控序列为元件构建了串联型YFP重组转染载体pH4-YFP-YFP-ACT。将构建的载体经电穿孔法转染柔嫩艾美球虫子孢子,并在MDBK细胞中进行培养。结果显示,在荧光显微镜下可以观察到黄色荧光。提示,串联型YFP可以在柔嫩艾美球虫中瞬时表达。
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| 关 键 词： | 柔嫩艾美球虫 黄色荧光蛋白 瞬时转染 |
Construction of tandem yellow fluorescent protein expression vector and its transient expression in Eimeria tenella |
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| LI Ji-dong,,HAO Li-li,,LIU Xian-yong,ZHOU Xiao-yan,SUO Xun. Construction of tandem yellow fluorescent protein expression vector and its transient expression in Eimeria tenella[J]. Chinese Veterinary Science, 2010, 40(1) |
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| Authors: | LI Ji-dong HAO Li-li LIU Xian-yong ZHOU Xiao-yan SUO Xun |
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| Affiliation: | LI Ji-dong1,2,HAO Li-li2,3,LIU Xian-yong2,ZHOU Xiao-yan2,SUO Xun2(1.College of Agriculture,Ningxia University,Yinchuan 750021,China,2.College of Veterinary Medicine,China Agricultural University,Beijing 100193,3.Institute of Pathogen Biology,Chinese Academy of Medical Sciences,Beijing 100730,China) |
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| Abstract: | To explore the transient expression of yellow fluorescent protein(YFP) in Eimeria tenella sporozoites,an eukaryotic vector pH4-YFP-YFP-ACT,which contains tandem repeated YFP,upstream flanking sequence of Histone4(H4) and downstream regulatory sequence of Actin both derived from E.tenella genomic DNA,was constructed.The sporozoites of E.tenella were electroporated with this expression vector and then cultured in MDBK cells lines.During the course of parasite development,YFP within parasites was observed unde... |
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| Keywords: | Eimeria tenella yellow fluorescent protein transient transfection |
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