| 禽劳斯肉瘤病毒群特异性抗原基因gp27片段的克隆及序列测定 |
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| 引用本文： | 宋建臣,关云涛,刘怀然,陈洪岩,刘胜旺,蔡雪辉,夏长友. 禽劳斯肉瘤病毒群特异性抗原基因gp27片段的克隆及序列测定[J]. 中国兽医科学, 2003, 33(11): 21-24 |
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| 作者姓名： | 宋建臣 关云涛 刘怀然 陈洪岩 刘胜旺 蔡雪辉 夏长友 |
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| 作者单位： | 1. 延边大学,农学院,动物医学系,吉林,龙井,133400
2. 中国农业科学院,哈尔滨兽医研究所,黑龙江,哈尔滨,150001 |
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| 摘 要： | 以RT PCR方法从人工感染禽劳斯肉瘤病毒 (Roussarcomavirus ,RSV )SR RSV的SPF雏鸡体内及SPF鸡胚成纤维细胞 (CEF)培养物中分别扩增出 72 0bp的 gp2 7片段 ,将RT PCR产物重组到质粒载体 pUC19中 ,并对重组质粒进行酶切分析及序列测定。结果表明 ,克隆片段为完整的目的基因 ,该片段的核苷酸序列与国外分离株RSVJ0 2 342的同源性达 97.3%。
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| 关 键 词： | 禽劳斯肉瘤病毒 gp27基因 基因克隆 序列测定 |
| 文章编号： | 1000-6419(2003)11-0021-04 |
| 修稿时间： | 2003-06-16 |
Cloning and sequence analysis of group specific antigen gp27 gene of rous sarcoma virus |
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| SONG Jian-chen,GUAN Yun-tao,LIU Huai-ran,CHEN Hong-yan,LIU Sheng-wang,CAI Xue-hui,XIA Chang-you. Cloning and sequence analysis of group specific antigen gp27 gene of rous sarcoma virus[J]. Chinese Veterinary Science, 2003, 33(11): 21-24 |
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| Authors: | SONG Jian-chen GUAN Yun-tao LIU Huai-ran CHEN Hong-yan LIU Sheng-wang CAI Xue-hui XIA Chang-you |
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| Affiliation: | SONG Jian-chen~1,GUAN Yun-tao~2,LIU Huai-ran~2,CHEN Hong-yan~2,LIU Sheng-wang~2,CAI Xue-hui~2,XIA Chang-you~2 |
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| Abstract: | The gp27 gene of 720 bp was amplified by RT-PCR method from SPF chicken and Chicken Embyo Fibroblast (CEF) which were infected with Rous sarcoma virus (RSV), and the gp27 gene was cloned into a plasmid veetor pUC19, the recombined plasmid were analyzed by restriction enzyme digestion and sequence analysis, the result showed that the cloning gene was the full-length target gene which has a 97.7% homology with foreign isolate strains RSV(J02342). |
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| Keywords: | Rous sarcoma virus gp27 gene gene cloning sequence analysis |
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