Discordance at D3S1358 locus involving SGM Plus™ and the European new generation multiplex kits |
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Authors: | Aliza RazielCarla Oz,Aviva Dell&rsquo Ariccia CarmonRafi Ilsar,Ashira Zamir |
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Affiliation: | a DNA Database Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q., Jerusalem 91906, Israel b Agentek (1987) Ltd., Atidim Scientific Park Building 5, Tel Aviv, Israel |
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Abstract: | During the course of routine database sample analysis in the Israel Police DNA database, an off-ladder D3S1358 allele, calculated to be >22.1, extending into the adjacent vWA locus was observed using Applied Biosystems SGM Plus™ kit.To verify the size of this D3S1358 long allele and to ensure it was not part of a trialle pattern in the neighboring locus, the sample was amplified using three of the European new generation STR multiplex kits: NGMTM (Applied Biosystem), Powerplex™ ESX and ESI (Promega). The results of these amplifications determined the variant to be a 22 allele. Subsequent sequencing confirmed this designation and revealed a nucleotide polymorphism. Ten additional SGM Plus™ profiled samples with D3S1358 alleles larger than 19, were re-analyzed using NGMTM and Powerplex™ ESX which also showed discordance in the calculated results between original SGM Plus™ designations and those obtained with the European new generation multiplexes. |
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Keywords: | Forensic science D3S1358 Discordance DNA database Multiplex kits DNA sequencing STR Variant allele |
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