|
|||||
|
|
RNA/DNA co-analysis from blood stains—Results of a second collaborative EDNAP exercise |
|
| Authors: | C. Haas,E. HansonM.J. Anjos,W. Bä rR. Banemann,A. BertiE. Borges,C. BouakazeA. Carracedo,M. CarvalhoV. Castella,A. ChomaG. De Cock,M. Dö tschP. Hoff-Olsen,P. JohansenF. Kohlmeier,P.A. LindenberghB. Ludes,O. Maroñ asD. Moore,M.-L. MorerodN. Morling,H. Niederstä tterF. Noel,W. ParsonG. Patel,C. PopielarzE. Salata,P.M. SchneiderT. Sijen,B. Svie?enaM. Turanská ,L. Zatkalí ková J. Ballantyne |
| Affiliation: | a Institute of Legal Medicine, University of Zürich, Switzerland b National Center for Forensic Science, University of Central Florida, Orlando, USA c Netherlands Forensic Institute, The Hague, The Netherlands d Forensic Science Service, Birmingham, UK e Institute of Forensic Science, Department of Criminalistic Biology and Genetic Analysis, Bratislava, Slovakia f Institute of Legal Medicine, University of Strasbourg, France g Reparto Investigazioni Scientifiche di Roma, Rome, Italy h Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, Denmark i Bundeskriminalamt, Wiesbaden, Germany j Institute of Forensic Medicine, University of Oslo, Norway k Institute of Legal Medicine, Innsbruck Medical University, Austria l Institute of Forensic Sciences, Ministry of the Interior, Department of Biology and DNA analysis, Slovenská Lupca, Slovakia m Forensic Genetic Service, Centre Branch, National Institute of Legal Medicine, Portugal o Institute of Legal Medicine, University Hospital, University of Cologne, Germany p Institut National de Police Scientifique, Laboratoire de Police Scientifique de Lyon, France q Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Spain r LGC Forensics, Middlesex, UK s National Institute for Criminalistics and Criminology, Brussels, Belgium t Unité de génétique forensique, Centre universitaire romand de médecine légale, Lausanne et Genève, Switzerland |
| Abstract: | A second collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling was organized by the European DNA Profiling Group (EDNAP). Six human blood stains, two blood dilution series (5-0.001 μl blood) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by the participating laboratories using a RNA/DNA co-extraction or solely RNA extraction method. Two novel mRNA multiplexes were used for the identification of blood: a highly sensitive duplex (HBA, HBB) and a moderately sensitive pentaplex (ALAS2, CD3G, ANK1, SPTB and PBGD). The laboratories used different chemistries and instrumentation. All of the 18 participating laboratories were able to successfully isolate and detect mRNA in dried blood stains. Thirteen laboratories simultaneously extracted RNA and DNA from individual stains and were able to utilize mRNA profiling to confirm the presence of blood and to obtain autosomal STR profiles from the blood stain donors. The positive identification of blood and good quality DNA profiles were also obtained from old and compromised casework samples. The method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise involving a RNA/DNA co-extraction strategy support the potential use of an mRNA based system for the identification of blood in forensic casework that is compatible with current DNA analysis methodology. |
| Keywords: | Forensic science Body fluid identification Blood RNA/DNA co-extraction EDNAP exercise mRNA profiling |
| 本文献已被 ScienceDirect 等数据库收录！ | |