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| 大鼠不同程度脑挫伤后caspase-3表达的研究 | |
| 引用本文： | Tao LY,Chen XP,Bian SZ. 大鼠不同程度脑挫伤后caspase-3表达的研究[J]. 法医学杂志, 2004, 20(1): 9-12 |
| 作者姓名： | Tao LY Chen XP Bian SZ |
| 作者单位： | 1. 苏州大学医学院法医学教研室,江苏,苏州,215007 2. 中国医科大学法医学院,辽宁,沈阳,11000l 3. 中国人民解放军第二0二医院法医学教研室,辽宁,沈阳,110001 |
| 摘 要： | 目的阐明不同程度及不同时间脑挫伤后caspase-3表达的变化。方法应用免疫组织化学染色、Westernblot和RT-PCR方法对48例实验性脑挫伤大鼠的脑组织进行了检验,同时以3例非脑挫伤和3例假手术的脑组织做对照。结果伤后1hcaspase-3即有表达,24～48h达高峰,挫伤后14dcaspase-3仍维持较高水平。阳性细胞多集中于挫伤区及周围带皮质和脑实质内海马区,不同程度脑挫伤各组之间阳性细胞面积和灰度存在差异;损伤组caspase-3酶原裂解增加,不同程度脑挫伤各组之间存在一定的差异;损伤组cas鄄pase-3表达量明显高于对照组;不同程度脑挫伤组之间于各个时间段caspase-3mRNA表达未见差异。结论Caspase-3表达增加,可辅助诊断1h～14d的脑挫伤,caspase-3阳性细胞面积和灰度的变化及酶原裂解程度与脑挫伤程度有关。 |
| 关 键 词： | 脑挫伤 caspase-3 脑挫伤形成时间 脑挫伤程度 凋亡 |
| 文章编号： | 1004-5619(2004)01-0009-04 |
| 修稿时间： | 2003-03-17 |
The study on the expression of caspase-3 in experimental brain contusion in rats |
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| Tao Lu-yang,Chen Xi-ping,Bian Shi-zhong. The study on the expression of caspase-3 in experimental brain contusion in rats[J]. Journal of Forensic Medicine, 2004, 20(1): 9-12 | |
| Authors: | Tao Lu-yang Chen Xi-ping Bian Shi-zhong |
| Affiliation: | School of Medicine, Suzhou University, Suzhou 215007, China. luyangtao@suda.edu.cn |
| Abstract: | Objective To investigate the expression of caspase-3in different posttraumati c intervals and severity of brain injury.Methods The study examined brain tissue samples of slight(n=24),severe(n=24)brain injury and control(n=6)of rat,using i mmunohistochemical staining,western-blot and RT-PCR method.Results Up-regulating of caspase-3expression was found in tissue from traumatic brain injury compared with controls in early1hour after TBI,and lasted for14days.The gray degree and threshold area of caspase-3positive cells is different in different severity of brain injury.Conclusion The increasing of caspase-3expression indicates that TBI exists.The gray degree,threshold area of caspase-3positive cells and the cleava ge degree of pro-caspase-3have association with the severity of brain injury. |
| Keywords: | cerebral contusion casapse-3 post contusion intervals severity apoptosis |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |