| 应用多种STR技术对基因嵌合体进行亲权鉴定分析 |
| |
| 引用本文： | 林源,赵珍敏,李莉.应用多种STR技术对基因嵌合体进行亲权鉴定分析[J].中国司法鉴定,2011(6):35-37,53. |
| |
| 作者姓名： | 林源 赵珍敏 李莉 |
| |
| 作者单位： | 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海200063 |
| |
| 基金项目： | 中央级科研院所社会公益研究资助项目(GY0803) |
| |
| 摘 要： | 目的 查找嵌合基因的来源并进行父母和孩子的亲权鉴定.方法 采用Chelex-100法抽提基因组DNA,用复合扩增和荧光检测技术对STR、X-STR和Y-STR基因座进行分型.结果 父亲为XX,XY基因嵌合体,其能够提供给孩子必须的遗传基因.结论 父亲为被检孩子的生物学父亲.
|
| 关 键 词： | PCR复合扩增 荧光检测 STR X-STR Y-STR检测技术 基因嵌合体 |
Paternity Analysis of Gene Chimera with Multiple STR Loci |
| |
| LIN Yuan
,
ZHAO Zhen-min
,
LI Li.Paternity Analysis of Gene Chimera with Multiple STR Loci[J].Chinese Journal of Forensic Sciences,2011(6):35-37,53. |
| |
| Authors: | LIN Yuan ZHAO Zhen-min LI Li |
| |
| Institution: | LIN Yuan,ZHAO Zhen-min,LI Li(Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,Shanghai 200063,China) |
| |
| Abstract: | Objective To detect the source of the chimeric genes and perform paternity test with STR,X-STR and Y-STR techniques.Methods Genomic DNA was extracted by Chelex-100 method.STR,X-STR and Y-STR loci were typed by multiplex amplification and fluorescence detection.Results The gender gene of the detected father is the chimera of XX and XY.The detected father can provide the paternal gene to the detected child.Conclusion The detected father is the child's biological father. |
| |
| Keywords: | PCR amplification fluorescence detection STR X-STR Y-STR gene chimera |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
