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| 6+1STR试剂盒和电泳凝胶EX-Q20用于DNA快速检验 | |
| 引用本文： | Ping Y,Zhou HG,Xu Y,Xia ZF,Zheng WG. 6+1STR试剂盒和电泳凝胶EX-Q20用于DNA快速检验[J]. 法医学杂志, 2011, 27(6): 444-446. DOI: 10.3969/j.issn.1004-5619.2011.06.012 |
| 作者姓名： | Ping Y Zhou HG Xu Y Xia ZF Zheng WG |
| 作者单位： | 1. 上海市公安局物证鉴定中心 上海市现场物证重点实验室,上海200083;复旦大学 生命科学学院,上海 200433 2. 上海市公安局物证鉴定中心 上海市现场物证重点实验室,上海,200083 3. 无锡中德美联生物技术有限公司,江苏无锡,214174 |
| 摘 要： | 目的 建立快速个体识别STR分型方法.方法 取采集于FTA上的血样200份,经打孔仪取等量血痕分别使用6+1 STR试剂盒结合新型毛细管电泳凝胶EX-Q20进行快速电泳分析和使用SinofilerTM试剂盒结合POP4胶进行电泳分析,比较两者所耗费时间和结果的差异. 结果 6+1 STR试剂盒结合新型毛细管电泳凝胶能够... |
| 关 键 词： | 法医遗传学 短串联重复序列 个体识别 快速检验 |
Rapid DNA identification using 6+1 STR kit and EX-Q20 electrophoresis |
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| Ping Yuan,Zhou Huai-Gu,Xu Yan,Xia Zi-Fang,Zheng Wei-Guo. Rapid DNA identification using 6+1 STR kit and EX-Q20 electrophoresis[J]. Journal of Forensic Medicine, 2011, 27(6): 444-446. DOI: 10.3969/j.issn.1004-5619.2011.06.012 | |
| Authors: | Ping Yuan Zhou Huai-Gu Xu Yan Xia Zi-Fang Zheng Wei-Guo |
| Affiliation: | Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China. pingyuan803@gmail.com |
| Abstract: | Objective To establish a rapid STR genotyping method for individual identification.Methods Two hundred blood samples from FTA were collected.Equal amount of blood were collected by puncher and analyzed using two methods（6＋1 STR kit in combination with EX-Q20 electrophoresis and SinofilerTM kit in combination with POP4 electrophoresis）.Consuming time and results of two methods were compared.Results 6＋1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time.Conclusion 6＋1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate,reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases.It also can raise the work efficiency. |
| Keywords: | forensic genetics STR individual identification rapid identification |
| 本文献已被 维普 万方数据 PubMed 等数据库收录！ | |