| 生物检材中的PCR抑制物及其处理技术 |
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| 引用本文： | 胡清清,刘宇轩,何仕霞,马丽莉,马红杜,黄代新. 生物检材中的PCR抑制物及其处理技术[J]. 中国法医学杂志, 2014, 0(3): 243-246 |
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| 作者姓名： | 胡清清 刘宇轩 何仕霞 马丽莉 马红杜 黄代新 |
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| 作者单位： | 华中科技大学同济医学院法医学系; |
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| 摘 要： | DNA分型技术在刑事案件的侦破中已得到广泛应用,但不同来源的各种生物检材往往含有PCR抑制物,其对扩增反应的影响不容忽视。因此,DNA样本扩增前预处理就显得尤为重要。本文主要就PCR抑制物种类及其对PCR的抑制机制、PCR抑制物应对技术等相关研究进展进行简要综述,旨在为相关研究和应用提供参考。
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| 关 键 词： | 法医物证学 生物检材 PCR抑制物 |
Research progress on PCR inhibitors in the biological material and their treatment technology |
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| Hu Qingqing,Liu Yuxuan,He Shixia,Ma Lili,Ma Hongdu,Huang Daixin. Research progress on PCR inhibitors in the biological material and their treatment technology[J]. Chinese Journal of Forensic Medicine, 2014, 0(3): 243-246 |
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| Authors: | Hu Qingqing Liu Yuxuan He Shixia Ma Lili Ma Hongdu Huang Daixin |
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| Affiliation: | (Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China) |
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| Abstract: | DNA typing technology has been widely applied in the detection of criminal cases. However, different sources of biological samples often contain PCR inhibitors and their effects on the amplification reaction cannot be ignored Therefore, the pretreatment of DNA samples for PCR amplification is particularly important. In this paper, the species of PCR inhibition, the mechanisms of PCR inhibition, and the treatment technology of PCR inhibitors are reviewed, aimed to provide new ideas for the related research and application. |
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| Keywords: | forensic biological evidence biological sample PCR inhibitors |
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