死后血液流动对氯胺酮在家兔尸体内再分布的影响

目的观察分析静脉注射氯胺酮家兔死后血液流动对体内药物浓度再分布影响。方法雄性新西兰大白兔随机分为实验组2组（各24只）,对照组（8只）;实验组家兔经耳缘静脉注入40mg/kg氯胺酮,1.5h后处死,其中一组立即结扎主动脉,另一组不结扎;家兔尸体仰卧位室温下保存,分别于死后0、3、6、12、24、48、72和96h解剖并采取组织和体液样本;对照组静脉注射等量生理盐水,同样方法解剖取相同样本。所有样本采用GC/MS和GC-NPD法检测样品中氯胺酮含量。结果两个实验组家兔尸体放置96h内氯胺酮含量,除尿液各时间点与0h以及相邻时间点之间比较均有显著性差异（P〈0.05）外,两组家兔其余各类样本与0h以及相邻时间点比较均无显著性差异（P〉0.05）。除尿液外,各类样本两个实验组之间均无显著性差异（P〉0.05）;结扎组和不结扎组心血与外周血中氯胺酮含量比值分别为0.90~1.03和0.90~1.02。结论静脉注射氯胺酮家兔死后的血液流动不是体内氯胺酮发生再分布的主要机制。

Influence of postmortem blood flow on redistribution of ketamine in rabbits corpse

Objective To investigate the influence of postmortem blood flow on redistribution of ketamine in rabbit in vivo intravenous infusion. Methods Male rabbits were randomly divided into two experiment groups and one control group. The experiment group rabbits received 40mg/kg ketamine intravenously （iv） via the ear vein and were killed 1.5h later in order to simulate the state of complete distribution in the body. One experiment group rabbits were ligatured aortic immediately and another group rabbits were not ligatured. The rabbit corpses were left in a supine position at room temperature. Anatomy and samples of tissues and fluids of rabbits were performed at Oh, 3h, 6h, 12h, 24h, 48h, 72h and 96h postmortem. The control group rabbits received a compai＇able amount of saline and the corresponding samples were taken correspondingly as blank check. The ketamine concentrations in samples were determined using GC/MS and GC-NPD. Results When the experiment group rabbit corpses left in a supine position within 96h, the ketamine content in urine was significant difference compared with Oh and adjacent time point （ P 〈 0. 05 ）. The ketamine content in other tissues and fluids of rabbits were no significant difference compared with Oh and adjacent time point （ P 〉 0. 05 ）. There was no significant difference of ketamine level in tissues and fluids （ in addition to urine ） of rabbits during the rabbits corpses left 96h respectively between two experiment group rabbits（P 〉0. 05）. The ratio of ketamine concentration in cardiac blood and peripheral blood （C/P） in two experiment group rabbits were 0.90 - 1.03 and 0.90 - 1.02 respectively. Conclusion The postmortem blood flow may not be a principal mechanism on redistribution of ketamine in animal corpse.