New alleles and mutational events at 14 STR loci from different German populations |
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| Authors: | Dorit Becker, Klaus Bender, Jeanett Edelmann, Frank G tz, Lotte Henke, Sandra Hering, Carsten Hohoff, Karolin Hoppe, Michael Klintschar, Matthias Muche, Burkhard Rolf, Reinhard Szibor, Volker Weirich, Martin Jung,Werner Brabetz |
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| Affiliation: | Dorit Becker, Klaus Bender, Jeanett Edelmann, Frank Götz, Lotte Henke, Sandra Hering, Carsten Hohoff, Karolin Hoppe, Michael Klintschar, Matthias Muche, Burkhard Rolf, Reinhard Szibor, Volker Weirich, Martin Jung,Werner Brabetz |
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| Abstract: | The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci. |
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| Keywords: | DNA typing Short tandem repeat Primer binding site mutation Silent allele Mutation rate |
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