| 毛细管气相色谱法测定酱油中4-甲基咪唑的含量 |
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| 引用本文： | 李继民,王彦吉,邹宁,姚丽娟. 毛细管气相色谱法测定酱油中4-甲基咪唑的含量[J]. 中国法医学杂志, 2007, 22(3): 151-153 |
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| 作者姓名： | 李继民 王彦吉 邹宁 姚丽娟 |
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| 作者单位： | 1. 中国刑警学院法医系,辽宁,沈阳,110035
2. 中国人民公安大学,北京,100038 |
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| 摘 要： | 目的建立毛细管气相色谱法定量分析酱油中有毒成分4-甲基咪唑的方法。方法酱油样品在层析柱中用二氯甲烷洗脱,洗脱液浓缩后加入N,N-二甲基苯胺作为内标,采用DB-FFAP毛细管柱分离样品,氮磷检测器测定4-甲基咪唑含量。结果方法线性范围为4.9~1.5×102μg/L;检测限为0.16μg/L;标准加入0.0102mg和0.0602mg 4-甲基咪唑的平均回收率为97.25%和99.44%。结论本文方法具有操作简便、快速、准确等优点,可用于检验酱油中的4-甲基咪唑。
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| 关 键 词： | 法医毒物分析学 毛细管气相色谱法 4-甲基咪唑 酱油 |
| 文章编号： | 1001-5728(2007)03-0151-03 |
| 修稿时间： | 2006-03-27 |
Detemination of 4-Methylimidazol in soy sauce by capillary gas chromatography |
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| LI Ji-min,WANG Yan-ji,ZOU Ning,et al.. Detemination of 4-Methylimidazol in soy sauce by capillary gas chromatography[J]. Chinese Journal of Forensic Medicine, 2007, 22(3): 151-153 |
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| Authors: | LI Ji-min WANG Yan-ji ZOU Ning et al. |
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| Affiliation: | LI Ji-min,WANG Yan-ji,ZOU Ning,et al.Department of Forensic Medicine China Criminal Police University Shenyang 110035,China |
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| Abstract: | Objective A method for the determination of 4-methylimidazole in soy sauce by capillary gas chromatography was studies.Methods The method consisted of a methylene chloride to elution,followed by concentration of the eluate.N,N-Dimethylaniline(IS) was added in and GC analysis of the eluate,prior to GC analysis.The GC analysis was carried out by DB-FFAP capillary column and nitrogen phosphorus detector(NPD).Results The linear range was 4.9mg~1.5×102 mg/L and the limit of detection was 0.16 ug/L.The average recoveries were 97.25%and 99.44% by stand addition method in 0.0102mg and 0.0602mg 4-methylimidazole.Conclusion The method was simple,rapid and sensitive.A useful method for determining 4-methylimidazole in soy sauce was provided for forensic analysis. |
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| Keywords: | Forensic toxicology analysis Capillary gas chromatography 4-methylimidazole Soy sauce |
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