DNA芯片技术检测HLA-DRB1和ABO基因型

用DNA芯片技术检测HLA-DRB1-ABO基因型。根据HLA和ABO不同基因亚型的独特序列设计探针,制成分型芯片;待检测样品经PCR反应标记上荧光之后,与探针在芯片上进行杂交,通过对杂交产生的荧光信号值进行分析,确定样品DRB1位点和ABO位点的基因亚型。将这一方法应用于111份样本的HLA-DRB1-ABO基因分型并将部分样品进行基因测序。检测结果证明本实验研制的HLA-DR-ABO基因分型芯片可准确分辨出DRB1位点30个等位基因、ABO位点6种基因型。该方法分辨率高、特异性强、重复性好、操作简便,对比常规的PCR-SSP方法,HLA-DR-ABO基因芯片方法更为直观,并具有集成化优势,可以在一张芯片上同时检测HLA和ABO位点,并实现一张芯片多人份,不仅适用于法医学亲子鉴定和个体识别,亦可应用于移植配型、HLA相关疾病及人类遗传学研究。

HLA-DRB1 and ABO Genotyping Using DNA Chip Technology

Objective To establish a new method performed on an oligoneucleotide array for genotyping HLA DRB1 and ABO. Methods According, to the sequence of HLA-DRBI and ABO,HLA-DRB1-ABO genotyping chips were made,then the labeled PCR products were hybridized with them, The signals were scanned by the scanner 4000 and analyzed by the Imagene software. In theliexperiment, 111 samples were genotyped and some of samples sequenced. Results The HLA-DRB1-ABO genotyping chips we made are accurate and sensitive. Compared with PCR-SSP and PCR-SSO methods, the genotyping chip method is more explicit and has the advantage of integration. It can be applied not only in the paternity test and individual identification but also in tissue matching and gentics research.