The collaborative study on typing group-specific component by eight forensic science laboratories |
| |
| Authors: | S A Westwood D J Werrett |
| |
| Affiliation: | 1. Nephrology and Urology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;2. Local Health Unit N. 2 "Marca Trevigiana", Public Health Department, Treviso, Italy;3. Pediatric Congenital Hematologic Disorders Research Center, Research Institute for Children''s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran and Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran;4. Student Research Committee (SRC), Baqiyatallah University of Medical Sciences, Tehran, Iran;5. Trauma Research Center, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran;6. JC School of Public Health and Primary Care, Chinese University of Hong Kong, Hong Kong, China;7. Department of Radiology, Baqiyatallah University of Medical Sciences, Tehran, Iran;1. Department of Transfusion Medicine & Transplant Immunology, Jaypee Hospital, Sector, 128, Noida 201304, India;2. Department of Transfusion Medicine, Medanta, The Medicity, Gurugram, India;3. Department of Transfusion Medicine, Apollo Hospital, Kolkata, India;4. Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India 576104;5. Department of Nephrology, Institute of Kidney Diseases and Research Center, Ahmedabad, India;6. Department of Transfusion Medicine, Indaprastha Apollo Hospital, New Delhi, India;7. Department of Transfusion Medicine, Manipal Hospital, Jaipur, India;8. Department of Transfusion Medicine, All India Institute of Medical Sciences, Patna, India;1. Department of Transfusion Medicine Medanta-The Medicity, Sector-38, Gurgaon, India;2. Department of Clinical Microbiology Institute of Liver and Biliary Sciences, New Delhi, India;3. Department of Microbiology College of Life Sciences, Jiwaji University Gwalior, India;4. Medanta Institute of Education and Research Medanta-The Medicity, Sector-38, Gurgaon, India;5. Independent Laboratory Consultant, Chennai, Tamil Nadu, India;6. Laboratory Medicine, Thane West, Mumbai 400606, India |
| |
| Abstract: | This report describes a collaborative study on typing group-specific component (GC), conducted between the Central Research and Support Establishment and the forensic science laboratories of England, Wales and Northern Ireland. A population study (n=114) was performed. Fifty blood donors were selected to provide a distribution, slightly biased from normal, in favour of the GC 1F-1F and GC 1F-1S phenotypes. A protocol was devised for preparing large bloodstains. The strongest GC bands were obtained from the edge of a stain after the blood had been treated with K+/EDTA. Each laboratory received a representative portion of the large bloodstains for GC typing. Five of the eight laboratories correctly grouped all the bloodstains. No errors directly attitributable to the system were recorded in over 800 tests, indicating that GC in bloodstains can be typed reliably using the combination of isoelectric focusing in ultrathin narrow pH interval gels followed by immunofixation and silver staining. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
