| 人类DNA小卫星区域RNA探针制备及应用的研究(Ⅰ)──pGEM-4Z-MYO亚克隆的构建 |
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| 引用本文： | 李玉新. 人类DNA小卫星区域RNA探针制备及应用的研究(Ⅰ)──pGEM-4Z-MYO亚克隆的构建[J]. 中国法医学杂志, 1994, 0(3) |
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| 作者姓名： | 李玉新 |
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| 作者单位： | 白求恩医科大学法医学教研室!长春，130021 |
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| 摘 要： | 利用DNA重组技术，由pUC19－MYO质粒中获得MYO小卫星DNA探针片段，将其插入到具有RNA聚合酶启动子的pGEM－4Z质粒的多克隆位点，从而构建了pGEM－4Z一MYO亚克隆，为制备RNA探针并将其应用于DNA指纹检测，提高DNA指纹的敏感性和小卫星DNA多态性的检出率奠定了基础。
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| 关 键 词： | 小卫星DNA DNA重组技术 pGEM－4Z质粒 |
PREPARATION OF RNA PROBE OF MINISATELLITE REGION IN HUMAN DNA AND ITS PRACTICAL USE(Ⅰ)-CONSTRUCTION OF SUB-CLONE OF pGEM-4Z-MYO |
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| Li Yuxin. PREPARATION OF RNA PROBE OF MINISATELLITE REGION IN HUMAN DNA AND ITS PRACTICAL USE(Ⅰ)-CONSTRUCTION OF SUB-CLONE OF pGEM-4Z-MYO[J]. Chinese Journal of Forensic Medicine, 1994, 0(3) |
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| Authors: | Li Yuxin |
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| Abstract: | By means of DNA recombinant techniques, the fragment of MYO minisatellite DNA probe 0b-tained from plasmid pUC19 - MYO was inserted into plamsid pGEM - 4Z containing RNA poly-merase promotor, thus a sub-clone pGEM- 4Z-MYO was constructed then. It provided the RNAprobe for the DNA fingerprinting and laid a foundation for raising the sensitivity of detecting the poly-morphism of the minisatel1ite DNA. |
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| Keywords: | Minisatelite DNA DNA recombinant techniques Sub-clone |
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