| 大鼠脑挫伤后神经元一氧化氮合成酶表达的研究 |
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| 引用本文： | 陈龙,李中,顾云菊,赵子琴. 大鼠脑挫伤后神经元一氧化氮合成酶表达的研究[J]. 中国法医学杂志, 2001, 16(2): 69-72 |
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| 作者姓名： | 陈龙 李中 顾云菊 赵子琴 |
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| 作者单位： | 复旦大学医学院法医学教研室, |
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| 摘 要： | 为观察脑挫伤后NOS1的表达及其与损伤时程的关系 ,采用自制自由落体撞击法致大鼠右顶叶局灶性脑挫伤模型 ,于不同时间段处死大鼠后 ,进行NOS1的免疫组织化学及原位杂交法研究 ,结果发现NOS1及其mRNA表达水平与伤后经过时间有一定相关性 ,即随着伤后经过时间的延长 ,NOS1及其mRNA表达逐渐增加 ,并能较好地鉴别脑挫伤之死前伤和死后伤 ,可作为推断脑挫伤经过时间的参考指标之一。
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| 关 键 词： | 脑挫伤 损伤经过时间 一氧化氮合成酶 免疫组织化学 原位杂交 |
| 文章编号： | 1001-5728(2001)02-0069-04 |
| 修稿时间： | 2000-08-01 |
Study on the expression of nitric oxide synthase 1(NOS1)and mRNA after rat cerebral contusion |
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| CHEN Long,LI Zhong,GU Yunju,et al.. Study on the expression of nitric oxide synthase 1(NOS1)and mRNA after rat cerebral contusion[J]. Chinese Journal of Forensic Medicine, 2001, 16(2): 69-72 |
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| Authors: | CHEN Long LI Zhong GU Yunju et al. |
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| Affiliation: | CHEN Long,LI Zhong,GU Yunju,et al./Department of Forensic Medicine,Medical Center of Fudan University,Shanghai 200032 |
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| Abstract: | The experimental right cerebral contusion model was established in rats.To observe the relationship between the expression of Nitric oxide synthase 1(NOS1)and time elapsed after cerebral contusion,the expression of Nitric oxide synthase 1(NOS1)and mRNA was studied by immunohistochemistry and in situ hybridization at different intervals after cerebral injury.The results indicated that a positive relationship existed between the expression of NOS1 and NOS1 mRNA and the intervals elapsed after brain injury.This method also can be used to distinguish antemortem and postmortem injury rat′s cerebral contusion.So that,the expression of NOS1 and NOS1 mRNA is of great value for timing of brain injury. |
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| Keywords: | Cerebral contusion Timing of injury NOS1 Immunohistochemistry In situ hybridization |
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