STRtyper-10F/G联合CODIS系统鉴定突变三联体和二联体

目的 评估STRtyper-10F/G、CODIS系统联合应用在突变三联体和二联体亲子鉴定中的鉴定能力.方法 104例亲子鉴定样本,采用CODIS检测,其中3例三联体发现突变的案例以及101例二联体中73例认定和28例排除案例,分别再使用STRtyper-10F/G检测；统计认定和排除情况、比较单独使用和联合使用在排除关系案例中的表现和法医学参数(H、DP、PE、PIC、TPI).结果 3例存在突变基因的三联体案例加做STRtyper-10F/G未发现更多的矛盾基因座,PI值均大于10 000,可作出认定结论；73例二联体认定案例中13例PI不足10 000,加做STRtyper-10F/G系统后PI＞10 000,可确认认定；28例排除案例最高排除率CODIS为50.00％、STRtyper-10F/G为64.29％；两个体系均具有较高的杂合度(H≥0.7)和信息量(PIC ＞0.7),联合应用CEP为0.999 999 999 505 3.结论 STRtyper-10F/G和CODIS系统联合应用可满足突变三联体和二联体亲子鉴定的需要.

The evaluation of STRtyper-10F/GTM and CODIS in triplet cases with inconsistent-loci and duo-paternity identification cases

Objective To evaluate the application value of the STR typer-10F/GTM combined with CODIS(combined DNA index system,CODIS) in triplet cases with inconsistent-loci and duo-paternity identification cases.Methods 3 cases of triplet with inconsistent-loci and 101 cases of duo-paternity identification were detected by using STRtyper-10F/GTM and CODIS.The paternity inclusion and exclusion of the STRtyper-10F/GTM and CODIS were analysed.Separate system and joint systems were compared on forensic parameters(H,DP,PE,PIC,TPI) and exclusive cases.Results After tested by STRtyper-10F/GTM,no inconsistent-loci were further found in 3 triplet cases with inconsistent-loci,and all of the paternity Index(PI) were above 10 000.The inclusion of paternity could be made.When detected only by CODIS,the PI of 13 duo-paternity cases was less than 10 000.After combined with the STRtyper-10F/GTM,all of their PI were above 10 000,and the inclusion of paternity could be made.For the 28 exclsive cases,the highest exclusion rates of the CODIS and STRtyper-10F/G were 50.00% and 64.29% respectively.Both systems had high heterozygosity(H≥ 0.7) and polymorphic information content(PIC>0.7),and the cumulative probability of exclusion(CEP) of the STR typer-10F/GTM combined with CODIS was 0.999 999 999 505 3.Conclusion The STRtyper-10F/GTM and CODIS systems are powerful and reliable for triplet cases with inconsistent-loci and duo-paternity identification cases.