华蟾素注射液对胃癌细胞MGC-803增殖、迁移和侵袭能力及上皮-间质转化的影响

目的 探究华蟾素(cinobufagin, CBG)注射液对人胃癌细胞MGC803增殖、迁移和侵袭能力以及上皮-间质转化(epithelial-mesenchymal transition, EMT)的影响。方法 CCK-8检测CBG注射液对MGC-803存活率的影响；倒置显微镜下观察CBG注射液对MGC-803形态的影响；采用细胞划痕实验和Transwell小室检测CBG注射液对胃癌细胞迁移和侵袭能力的影响；Western blot检测人胃癌细胞MGC-803中E-cadherin、N-cadherin、Vimentin和Snail蛋白表达水平。结果 华蟾素注射液能显著抑制MGC-803细胞的增殖、迁移与侵袭(P<0.05),且呈剂量依赖性。Western blot检测结果显示，CBG注射液能显著上调E-cadherin蛋白表达水平(P<0.05),显著下调N-cadherin、Vimentin、Snail蛋白表达水平(P<0.05)。结论 华蟾素注射液可以抑制胃癌细胞MGC-803的增殖、迁移侵袭及EMT。

Effect of Cinobufagin Injection on the Proliferation, Migration, and Invasion Abilities and Epithelial-Mesenchymal Transition of Gastric Cancer MGC803 Cells

Objective To investigate the effect of cinobufagin (CBG) injection on the proliferation,migration,and invasion abilities and epithelial-mesenchymal transition (EMT) of human gastric cancer MGC803 cells.Methods CCK-8 assay was used to observe the effect of CBG injection on the survival rate of MGC803 cells;the effect of CBG injection on the morphology of MGC803 cells was observed under an inverted microscope; wound healing assay and Transwell assay were used to investigate the effect of CBG injection on the migration and invasion abilities of gastric cancer cells;Western blot was used to measure the protein expression levels of E-cadherin,N-cadherin,Vimentin, and Snail in human gastric cancer MGC803 cells.Results CBG injection significantly inhibited the proliferation, migration, and invasion of MGC803 cells in a dose-dependent manner.Western blot showed that CBG significantly upregulated the protein expression level of E-cadherin (P<0.05) and downregulated the protein expression levels of N-cadherin,Vimentin,and Snail (P<0.05). Conclusion CBG injection can inhibit the proliferation, migration,invasion,and EMT of gastric cancer MGC803 cells.