| SPME与GC/MS相结合检验尿液中的氯胺酮 |
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| 引用本文： | 应剑波,张迪. SPME与GC/MS相结合检验尿液中的氯胺酮[J]. 中国法医学杂志, 2005, 20(6): 342-344 |
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| 作者姓名： | 应剑波 张迪 |
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| 作者单位： | 浙江省杭州市公安局刑侦支队,浙江,杭州,310004 |
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| 摘 要： | 目的应用固相微萃取与气相色谱-质谱联用技术分析尿液中的氯胺酮。方法对影响SPME萃取效果的萃取头类型、萃取时间、解析时间、离子强度等因素条件进行优化,分析尿中氯胺酮。结果在0.5~2.5μg/m l范围内线性关系良好,线性相关系数为0.995 6;最小检出限为2.5μg/L。结论该方法具有预处理简单、分析速度快、灵敏度高等优点,适合实际案件尿样的检验。
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| 关 键 词： | 法医毒物分析 固相微萃取 气相色谱 质谱 氯胺酮 尿液 |
| 文章编号： | 1001-5728(2005)06-0342-03 |
| 修稿时间： | 2004-11-22 |
Determination of Ketamine in Urine by Solid Phase Microextraction Coupled with GC Chromatography-Mass Spectrometry ( GC/MS ) |
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| Ying Jian-bo,Zhang Di. Determination of Ketamine in Urine by Solid Phase Microextraction Coupled with GC Chromatography-Mass Spectrometry ( GC/MS )[J]. Chinese Journal of Forensic Medicine, 2005, 20(6): 342-344 |
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| Authors: | Ying Jian-bo Zhang Di |
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| Affiliation: | (Ying Jian-bo,Zhang Di./Punlic Security Bureau of Hangzhou,Hangzhou 310004,China) |
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| Abstract: | Objective A rapid methed for the determination of ketamine in urine by solid phase microextraction(SPME) coupled with GC/MS was developed.Methods Some factors influencing SPME extraction efficiency such as ionic strength of solutions,extraction temperatare desorption time were studied.The extraction effects among three fiber coatings,polydimethylsiloxane(PDMS) and Polyacrylate(PA) and carbowax/divinglbenzene,CW/DVB,on ketamine were compared.The operating conditions of GC-MS were also investigated.Results The linearity were good in the range of 0.5-2.5μg/ml,the linearity correlation coefficient was 0.9956,and The detection limit of ketamine was in the range of 2.5μg/L.Conclusion: It indicates that solid phase micro-extraction is simple,fast,sensitive and no contaminative method.The methods reported in this paper are proper for case examination. |
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| Keywords: | Forensic toxicology analysis Solid phase microextraction Gas chromatography-mass spectrometry Ketamine Urine |
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