Simultaneous diagnosis of coagulation factor XIIA (F13A) and plasminogen (PLG) phenotypes by polyacrylamide gel isoelectric focusing. |
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Authors: | J L Caeiro C Llano E Parra |
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Affiliation: | Departamento de Antropoloxía, Facultade de Bioloxía, Universidade de Santiago de Compostela, Galicia, Spain. |
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Abstract: | In this paper, we report a simple rapid method for simultaneous determination of Coagulation Factor XIIIA (F13A) and plasminogen (PLG) phenotypes by PAGIF with a nominal pH range of 3.5 to 10, followed by immunofixation and silver stain. Critical considerations concerning the conditions of molecular separation and detection strategies are also presented. |
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