| 多重置换扩增技术用于法医学微量DNA检测效果 |
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| 引用本文： | 陈玲,刘超,王慧君,邱平明. 多重置换扩增技术用于法医学微量DNA检测效果[J]. 法律与医学杂志, 2008, 0(6) |
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| 作者姓名： | 陈玲 刘超 王慧君 邱平明 |
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| 作者单位： | 南方医科大学法医学系;广州市刑事科学技术研究所; |
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| 基金项目： | 公安部应用创新计划项目(编号:2005YYCX57136);;广州市重大科技攻关项目(编号:2005Z1-E0051) |
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| 摘 要： | 目的探讨多重置换扩增(MDA)技术对法医学微量DNA样品STR检测分型的效果。方法用MDA技术对不同模板量DNA进行全基因组扩增(WGA),扩增产物用实时荧光定量PCR技术定量、用Profiler PlusTM试剂盒检测基因型。结果该方法可对模板DNA增加104～106倍。1ng样品DNA的MDA产物可获得9个STR基因座和Amelogenin性别基因座的准确分型结果;低于0.1ng的样品DNA经MDA扩增后,基因座检出数增加,但可见等位基因不平衡或丢失现象。结论MDA技术可有效增加DNA模板量和提高微量DNA分型效果。但样品DNA量低于0.1ng时,MDA产物的STR分型结果判读须慎重。
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| 关 键 词： | 多重置换扩增 全基因组扩增 微量DNA检测 |
Effect of multiple displacement amplification on forensic minute DNA analysis |
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| Chen Ling. Effect of multiple displacement amplification on forensic minute DNA analysis[J]. The Journal of Law & Medicine, 2008, 0(6) |
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| Authors: | Chen Ling |
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| Affiliation: | Chen Ling.Institute of Forensic Medicine,Southern Medical University,Guangzhou 501515 |
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| Abstract: | Objective To study the effect of multiple displacement amplification(MDA) on forensic minute DNA analysis.Methods Whole genome amplification(WGA) was performed for various amount of sample DNA,and the amplified products were quantitated by real-time quantitative PCR and genotyped by Profiler PlusTM kit.Results The template DNA can be amplified 104 to 106 folds by MDA.Using this technology,all 9 STR loci and Amelogenin gene can be correctly genotyped by MDA from 1 ng genomic DNA.When the genomic DNA is not m... |
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| Keywords: | Multiple displacement amplification Whole genome amplification Minute DNA analysis |
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