首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Evaluation and applicability of Alere iCup DX 14 for rapid postmortem urine drug screening at autopsy
Authors:Steven Towler BS  Marta Concheiro PhD  Sue Pearring MS  Luke N Rodda PhD
Institution:1. Department of Sciences, John Jay College of Criminal Justice, City University of New York, New York, NY, USA;2. Forensic Laboratory Division, Office of the Chief Medical Examiner, San Francisco, CA, USA;3. Department of Laboratory Medicine, University of California, San Francisco, CA, USA;4. Luke N. Rodda, Forensic Laboratory Division, Office of the Chief Medical Examiner, 1 Newhall Street, San Francisco, CA, USA.;5. Email: luke.rodda@sfgov.org
Abstract:Performing point‐of‐care urine drug screen testing at autopsy by a forensic pathologist may provide an early indication of the presence of analytes of interest during autopsy. An evaluation for the screening of 14 classes of common drugs of abuse in postmortem urine by the point‐of‐care screening device, Alere iCup DX 14, is presented. One hundred ninety postmortem urine samples were screened with the iCup occurring at autopsy by the forensic pathologist. Positive and negative results obtained from the screening kit were evaluated against confirmatory test results obtained using routine forensic toxicology analyses that employed LC‐MS/MS and GC‐MS to detect a combination of over 85 common drugs of abuse and medications. Sensitivity for each respective iCup drug class ranged from 66% (buprenorphine) to 100% (methadone, tricyclic antidepressants). Specificity for each respective iCup drug class ranged from 89% (benzodiazepines) to 100% (amphetamines, barbiturates, buprenorphine, 3,4‐methylenedioxymethamphetamine, methadone). Positive predictive values ranged from 44% (benzodiazepines) to 100% (amphetamines, barbiturates, buprenorphine, methylenedioxymethamphetamine, methadone), while negative predictive values ranged from 96% (methamphetamine) to 100% (barbiturates, methadone, tricyclic antidepressants). A high false‐positive rate was yielded by the benzodiazepine class. The lack of fentanyl screening in the point‐of‐care device is a significant limitation considering its prolific prevalence in forensic casework. The results obtained in the study should be acknowledged when considering the use of the Alere iCup DX 14 in the context of postmortem casework to help indicate potential drug use contemporaneously with autopsy and when requiring such preliminary results prior to the release of a final forensic toxicology report.
Keywords:autopsy  drug screen  forensic toxicology  immunoassay  point‐of‐care testing
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号