GC-MS分析尿液中甲卡西酮

目的建立尿液中甲卡西酮的气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)分析方法。方法在尿液中加入内标双苯戊二氨酯(SKF525A)和pH=9的缓冲溶液,用乙酸乙酯提取,提取液在50℃氮气流下挥干,残余物用甲醇溶解,用GC-MS分析。结果尿液中甲卡西酮在0.02~2.00μg/m L质量浓度范围内线性关系良好,线性方程为y=0.301 9 x+0.018 9(r=0.999 2),检出限为0.01μg/m L。尿液中甲卡西酮回收率为96.4%~99.2%,日内精密度为5.8%~7.6%,日间精密度为6.0%~8.1%。结论该方法操作简便、灵敏度高,可用于司法鉴定实践尿液样品中甲卡西酮的分析。

Determination of Methcathinone in Urine by GC-MS

Objective To establish a method for the analysis of methcathinone in urine by gas chromatography-mass spectrometry (GC-MS).Methods Proadifen hydrochloride (internal standard) and buffer solution (pH=9) were added into the urine samples,and methcathinone was extracted by ethyl acetate.The extract was volatilized in 50 ℃ nitrogen gas flow and the remnant was dissolved by methanol and analysed by GC-MS.Results The methcathinone in urine showed a good linear relationship in the mass concentration range of 0.02-2.00 μg/mL.The linear equation was y=0.301 9x+0.0189 (r=0.9992),and the detection limit was 0.01 μg/mL.The recoveries of methcathinone in urine was 96.4％-99.2％,with the intraday precision of 5.8％-7.6％ and the inter-day precision of 6.0％-8.1％.Conclusion The method is convenient and sensitive,which can be applied to the forensic identification of methcathinone in urine.