脑外伤后短时间内死亡尸体脑干病理变化

建立脑干机械性损伤（肉眼无明显改变）致迅速死亡的动物模型，应用免疫组化、分子生物学、电镜及图像分析等方法，研究脑千损伤的物质基础——神经元、轴索、胶质细胞、血脑屏障。研究结果表明：脑外伤后短时内死亡是脑干损伤的结果，白蛋白、纤维联结蛋白、神经微丝蛋白、胶质纤维酸性蛋白、原癌基因c－Fos等可作为诊断脑干损伤早期病变的指标。在实验研究的基础上，将上述指标应用于怀疑脑干损伤致死案例，验证了这些指标的实用价值，以期为脑外伤后短时间内死亡案例的死因判定提供客观证据，为颅脑损伤的临床救治工作指明重点。

Study on pathological changes of brain-stem taken from corpses of traumatic brain iujury

Experimental mechanical brain injury model of rats died within short intervalafter head injury had been established. Changes of neurons, axons, glias cell, and brood-brainbarrier in brain stems were studied by using immunohistochemistry, molecular biology -method, electron microscope and image analysis. The results showed that the cause of deathwas the BSI. A1, Fn, NF, GFAP, and c-fos could .be used as the sensitive diagnostic cri-teria. The established methods for diapnosis of BSI were applied to the forensic medicinepractice.