| D6S2418在中国（汉族）、泰国和德国人群中的遗传多态性 |
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| 引用本文： | 刘志勇,陈国弟,任广田,余琳,韩军鸽,王晶,廖淼,梁伟波,张林. D6S2418在中国（汉族）、泰国和德国人群中的遗传多态性[J]. 法医学杂志, 2005, 21(4): 283-285 |
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| 作者姓名： | 刘志勇 陈国弟 任广田 余琳 韩军鸽 王晶 廖淼 梁伟波 张林 |
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| 作者单位： | 1. 四川大学华西基础与法医学院法医物证教研室,四川,成都,610041
2. 深圳市公安局盐田分局,广东,深圳,518082 |
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| 基金项目： | 国家自然科学基金资助项目(30171033) |
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| 摘 要： | 目的获得D6S2418基因座的群体遗传学数据,分析其基因频率在不同群体间的分布情况是否存在差异,并分析其在法医学中的应用价值。方法采用PCR、非变性聚丙烯酰胺凝胶电泳(PAGE)及银染技术分析中国成都地区汉族、泰国曼谷地区泰国人群以及德国Maint地区德国人群中D6S2418基因座的遗传多态性,获得三个群体D6S2418基因座的群体遗传学数据。结果从300份分别采自成都地区汉族、泰国曼谷地区泰国人群以及德国Maint地区德国人群三个群体的无血缘关系个体的静脉血,共发现9个等位基因,观测到31种基因型。观测杂和度为64%～81%,个人识别机率为84.2%～93.5%,经统计学检验,基因型的频率分布符合Hardy-Weinberg平衡定律。等位基因频率的分布在三个群体间有显著差异。结论D6S2418基因座的个人识别能力高,在法医学个人识别和亲子鉴定应用中有较高价值。
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| 关 键 词： | 短串联重复序列 PCR D6S2418 遗传多态性 |
| 文章编号： | 1004-5619(2005)04-0283-03 |
| 修稿时间： | 2005-01-05 |
Research on the Genetic Polymorphisms of D6S2418 Locus in Chinese Han Ethnic, Thai and Germany Populations |
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| LIU Zhi-yong,CHEN Guo-di,REN Guang-tian,et al.. Research on the Genetic Polymorphisms of D6S2418 Locus in Chinese Han Ethnic, Thai and Germany Populations[J]. Journal of Forensic Medicine, 2005, 21(4): 283-285 |
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| Authors: | LIU Zhi-yong CHEN Guo-di REN Guang-tian et al. |
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| Affiliation: | LIU Zhi-yong1,CHEN Guo-di1,REN Guang-tian2,et al. |
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| Abstract: | Objective To obtain the genetic data on STR locus D6S2418,and analyzed its genetic polymorphism in 3 different races (Han,Thai and Germany). Methods The blood samples from 300 unrelated individuals were analyzed by PCR,PAG electrophoresis and silver staining. Results 9 alleles and 31 genotypes were observed.The observed heterozygosity and the discrimination power were 64%-81% and 84.2%-93.5%,respectively.The genotype distributions of D6S2418 locus in 3 populations were in accordance with Hardy-Weinberg equilibrium and the difference of allelic frequency distributions among 3 populations was obvious. Conclusion The methods founded are easy to perform and sensitive. The discrimination power and exclusion chances of this locus are desirable for forensic analysis and application. |
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| Keywords: | short tandem repeat PCR D6S2418 genetic polymorphism |
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