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| 人脑挫伤后GFAP,PCNA的法医病理学研究 | |
| 引用本文： | Tao L,Wao D,Liu P,Yu S,Hao C,Wu X. 人脑挫伤后GFAP,PCNA的法医病理学研究[J]. 法医学杂志, 2000, 16(3): 137-138,140 |
| 作者姓名： | Tao L Wao D Liu P Yu S Hao C Wu X |
| 作者单位： | 1. 中国医科大学,辽宁沈阳,110001 2. 沈阳刑事科学技术研究所,辽宁,110002 3. 辽宁中医学院,辽宁沈阳,110031 |
| 摘 要： | 采用在实际工作中所遇到的脑挫伤案例标本 ,利用免疫组织化学染色 ,观察人脑挫伤后GFAP ,PCNA的改变 ,用图象分析仪对免疫组织化学染色阳性细胞的面积和灰度等进行定量测量 ,EpiInfo统计软件分析 ,得出人脑挫伤后GFAP ,PCNA变化的时间性规律 ,为脑挫伤形成时间推断 ,提供新的形态学依据。GFAP阳性细胞面积、灰度伤后24h即有显著增加 (P<0 01) ,7天达高峰 (P<0 01) ,以后有逐渐下降的趋势 ;PCNA阳性细胞面积、灰度于伤后4天达到最大值 (P<0 01) ,以后逐渐下降。因此 ,伤后人脑组织GFAP、PCNA免疫组织化学染色阳性细胞面积、灰度改变有时间规律 ,GFAP、PCNA可作为2天到20天间不同时间脑损伤的推断指标。 |
| 关 键 词： | 脑挫伤 免疫组织化学 图象分析 |
The changes of GFAP and PCNA after human brain contusion: an immunohistochemical study in forensic pathology |
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| Tao L,Wao D,Liu P,Yu S,Hao C,Wu X. The changes of GFAP and PCNA after human brain contusion: an immunohistochemical study in forensic pathology[J]. Journal of Forensic Medicine, 2000, 16(3): 137-138,140 | |
| Authors: | Tao L Wao D Liu P Yu S Hao C Wu X |
| Affiliation: | Institute of Forensic Sciences of ShenYang, ShenYang 110001, PRC. |
| Abstract: | In order to find out changes of GFAP and PCNA after human brain trauma, samples of 38 cases were studied by the immunnohistochemistry method combined with quantitative image analysis The gray degree and area of PCNA positive cells increased to the maximum at the 4th day and then decreased gradually GFAP positive cells began to increase significantly 12 hours after contusion, reached the maximum at the 4th day and stayed in high level until the 7th day These results suggest that: (1) GFAP, PCNA show regular changes with survival time after brain contusion;(2) GFAP and PCNA can be used as time markers of brain contusion from 2-20 days |
| Keywords: | brain trauma immunnohistochemistry image analysis (Original article on page 137) |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |