| 小鼠皮肤切创愈合过程中c-jun的表达 |
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| 引用本文： | 杨玫,官大威,熊昌艳,程子惠,于天水. 小鼠皮肤切创愈合过程中c-jun的表达[J]. 法医学杂志, 2009, 25(6): 401-404. DOI: 10.3969/j.issn.1004-5619.2009.06.001 |
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| 作者姓名： | 杨玫 官大威 熊昌艳 程子惠 于天水 |
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| 作者单位： | 1. 中国医科大学,法医学院,辽宁,沈阳,110001
2. 中国医科大学,法医学院,辽宁,沈阳,110001;江苏大学,法医学教研室,江苏,镇江,212001 |
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| 基金项目： | 国家自然科学基金资助项目，教育部留学归国基金资助项目 |
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| 摘 要： | 目的探讨小鼠皮肤切创愈合过程中损伤区c-jun的表达及不同损伤时间c-jun的变化规律。方法应用免疫组织化学染色和Western印迹技术检测小鼠皮肤切创后各个时间段c-jun的表达变化情况。结果正常组织中有少量c-jun表达。伤后3～12h损伤区c-jun主要表达在中性粒细胞中,1～5dc-jun阳性细胞主要以单个核细胞和成纤维细胞为主,7～14dc-jun主要在成纤维细胞中表达。阳性细胞率3～12h逐渐增高,12h达高峰,1～5d有所下降,7d阳性细胞率达第二峰值,10～14d逐渐降低。Western印迹法检测显示各个时间段均有c-jun阳性条带,其中12h和7d为c-jun含量的2个高峰。结论小鼠皮肤切创愈合过程中c-jun在损伤区对诱导中性粒细胞、单个核细胞及成纤维细胞的凋亡可能起着重要作用,同时c-jun表达的规律性变化可用于损伤时间的推断。
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| 关 键 词： | 法医病理学 创伤和损伤 伤口愈合 基因,jun 损伤时间 小鼠 |
Expression of c-jun During the Incised Wound Healing in Mice Skin |
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| YANG Mei,GUAN Da-wei,XIONG Chang-yan,CHENG Zi-hui,YU Tian-shui. Expression of c-jun During the Incised Wound Healing in Mice Skin[J]. Journal of Forensic Medicine, 2009, 25(6): 401-404. DOI: 10.3969/j.issn.1004-5619.2009.06.001 |
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| Authors: | YANG Mei GUAN Da-wei XIONG Chang-yan CHENG Zi-hui YU Tian-shui |
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| Affiliation: | YANG Mei1,GUAN Da-wei1,XIONG Chang-yan1,2,CHENG Zi-hui1,YU Tian-shui1 (1. School of Forensic Medicine,China Medical University,Shenyang 110001,China,2. Department of Forensic Medicine,Jiangsu University,Zhenjiang 212001,China) |
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| Abstract: | Objective To investigate the time-dependent expression of c-jun during the healing of incised wound in mice skin. Methods The expression of c-jun in different stages after the incised wound were detected by immunohistochemistry and Western blot. Results There was a low level expression of c-jun in normal mice skin. Expression of c-jun was mainly detected in neutrophils from 3 h to 12 h after injury. The c-jun positive cells were almost mononuclear cells (MNCs) and fibroblasts between 1 d and 5 d after injur... |
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| Keywords: | jun |
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