| ABO基因分型的等位基因特异性引物消耗法 |
| |
| 引用本文： | 邵武,姜先华,于蛟,黄斌.ABO基因分型的等位基因特异性引物消耗法[J].中国法医学杂志,2007,22(2):85-86. |
| |
| 作者姓名： | 邵武 姜先华 于蛟 黄斌 |
| |
| 作者单位： | 辽宁省刑事科学技术研究所,辽宁,沈阳,110032 |
| |
| 摘 要： | 目的建立一种新的ABO基因型分型的等位基因特异性引物消耗法(CASPA)。方法根据ABO基因碱基序列中的第261、297、803nt3处多态性位点设计6条特异性引物及1条公用引物,采用CASPA法鉴定146名中国汉族无关个体血液斑样品的ABO基因型。结果146名中国汉族无关个体血液斑样品中检出AAb、AB、AO1、BOv、O1Ov、AA、BB、O1O1、BO1等9种基因型,结果明确,其基因频率分布符合Hardy-Weinberg平衡。结论ABO基因型CASPA分型方法为ABO血型的鉴定提供了一个新的检测方法。
|
| 关 键 词： | 法医物证学 ABO基因型 等位基因特异性引物消耗法 |
| 文章编号： | 1001-5728(2007)02-0085-03 |
| 修稿时间： | 2005年4月11日 |
A technique for ABO genotyping by consumed allele-specific primer analysis |
| |
| SHAO Wu,JIANG Xian-hua,YU Jiao,et al..A technique for ABO genotyping by consumed allele-specific primer analysis[J].Chinese Journal of Forensic Medicine,2007,22(2):85-86. |
| |
| Authors: | SHAO Wu JIANG Xian-hua YU Jiao |
| |
| Institution: | SHAO Wu,JIANG Xian-hua,YU Jiao,et al./Liaoning Forensic Science Institute,Shenyang 110032,China |
| |
| Abstract: | Objective To establish a new technique,known as consumed allele-specific primer analysis(CASPA),for ABO genotyping.Methods Six specific primers and one public primer were designed according to three polymorphism loci(261nt、297nt、803nt) in the nucleotide sequences of ABO gene.ABO genotypes of 146 unrelated Han Chinese were analyzed by CASPA.Results Nine ABO genotypes were differentiated by CASPA and their distributions were consistent with Hard-Weinberg equilibrium.Conclusion CASPA can be used as a new technique for ABO genotyping. |
| |
| Keywords: | Forensic biological evidence ABO genotype Consumed allele-specific primer analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
