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Chemical Analysis in the Corpus Callosum Following Traumatic Axonal Injury using Fourier Transform Infrared Microspectroscopy: A Pilot Study |
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| Authors: | Ji Zhang MD Liang Liu MD Jiao Mu MSc Tiantong Yang MD Na Zheng MD Hongmei Dong MD |
| Institution: | 1. Department of Forensic Medicine, Xi'an Jiaotong University, 74 West Yanta Road, Xi'an, Shanxi, China;2. Department of Forensic Medicine, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China;3. Collaborative Innovation Center of Judicial Civilization, Key Laboratory of Evidence Science, China Univerisy of Political Science and Law, 116 Lugu Road, Beijing, China;4. Department of Pathophysiology, Shenzhen Univeristy, 1688 Nanhai Road, Shenzhen, China;5. Department of Forensic Medicine, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China
Additional information and reprint requests: Hongmei Dong, M.D. Department of Forensic Medicine Huazhong University of Science and Technology 13 Hangkong Road Wuhan, Hubei, China E-mails: shanrenziyoumiao@126.com;6. hongmeidong1@hotmail.com |
| Abstract: | Evaluating traumatic axonal injury remains challenging in clinical and forensic sciences as its identification is difficult using routine diagnostic methods. This study used Fourier transform infrared microspectroscopy to detect TAI within the corpus callosum in an animal model. Protein conformational analysis revealed significantly increased β-sheet and β-turn contents paralleled by a decrease in α-helix content at 24 h postinjury, while the antiparallel β-sheet content was decreased at 12 h postinjury. Compared with the control group, the lipid/protein ratio was significantly reduced in all of the injured groups. At 24 h postinjury, there were increases in the olefinic=CH and CH3 group of lipids accompanied by the decreased CH2 group, but the results at 12 and 72 h were contrary to that at 24 h. Our study showed that FTIRM could differentiate injured from normal white matter at different time points following TBI via examination of these infrared spectral parameters. |
| Keywords: | forensic science forensic pathology traumatic axonal injury traumatic brain injury Fourier transform infrared microspectroscopy animal study |