反相高效液相色谱法检测生物胺的法医学应用初探

目的采用反相高效液相色谱法(RP-HPLC)检测大鼠肌肉组织中7种生物胺,并探讨其在法医学中的应用价值。方法以丹磺酰氯为衍生剂,通过ZORBAX Rx-C18反相色谱柱分离,以乙腈-水作流动相梯度洗脱,流速为1.0mL/min,柱温为20℃,DAD检测器,对色胺、腐胺、尸胺、组胺、酪胺、亚精胺、精胺7种生物胺进行检测。结果 7种生物胺得到良好分离,在0.5～100.0μg/mL的范围内线性良好,相关系数均>0.99,检出限在0.14～0.50μg/mL之间;对大鼠肌肉样本的添加回收率在83.8%～100.6%之间,相对标准偏差均<11%。结论采用RP-HPLC法检测生物胺,方法简便、快速、灵敏,能够满足尸体组织中生物胺检测的需要。

The preliminary study on forensic application for determination of biogenic amines by RP-HPLC

Objective An analytical method of RP-HPLC has been developed to assay biogenic amines in rat muscle tissue and to discuss its forensic application.Methods The samples were derivatized with dansyl chloride and then determined by RP-HPLC on the ZORBAX Rx-C18 reverse phase chromatographic columns.A gradient elution program with a mixture of acetonitrile and water was used at a flow rate of 1.0 mL/min.The diode array detector(DAD) was used and the column temperature was 20℃.Results Seven kinds of biogenic amines were separated completely and the standard curve was linear over the concentration range of 0.5~100.0μg/mL.The detection limits were 0.14~0.50μg/mL for 7 kinds of biogenic amines.The average recoveries were in the range of 83.8%~100.6% and the relative standard devitions were all less than 11%.Conclusion The method was convenient,rapid and sensitive,which could satisfy the deternination of biogenic amines in the organism.