冠状动脉粥样斑块中C-反应蛋白的免疫组化研究

目的探讨冠状动脉粥样硬化斑块中C-反应蛋白(CRP)对冠心病猝死(SCD)的诊断意义。方法从本教研室2001~2004年尸检案例中挑选68例案例资料和心脏标本,分为3组A组SCD(27例);B组冠心病非猝死者(21例);C组无明显动脉粥样硬化病变的死者(20例);应用免疫组化染色(SABC法)和图像分析技术,检测每例冠状动脉左前降支和右主支粥样硬化斑块内的CRP染色情况,并对所得数据进行统计分析。结果A组有20例CRP免疫组化染色强阳性,6例呈较强阳性,1例为弱阳性;B组中3例呈较弱阳性,11例微弱阳性,7例为阴性;C组均未见阳性反应。结论检测冠状动脉粥样硬化斑块中的CRP对SCD的死后诊断具有一定意义。

Immunohistochemical study of C-reactive protein in the atherosclerotic plaque of coronary artery

Objective To investigate the change of C-reactive protein (CRP) in the coronary atherosclerotic plaque and its diagnostic value to sudden coronary death (SCD).Methods Sixty-eight cases and heart samples were selected from the files in our department from 2001 to 2004, which were divided into 3 groups. Group A(27 cases)died of SCD; group B (20 cases), non-sudden death with coronary heart disease; group C(20 cases), any cause of death without atherosclerosis. CRP was detected by immunohistochemical technology (SABC method) and image analysis system.Results Of 27 cases in group A, there were 20 cases which showed strong positive expression; 6 cases, positive expression; and 1 case, weak positive expression. Of 21 cases in group B, 3 cases appeared weak positive expression; 11 cases, very weak positive expression; and 7 cases, negative reaction. No positive reaction of CRP was found in the 20 cases of group C. Conclusion The immunohistochemical detection of CRP in the coronary atherosclerostic plaque has some significance for diagnosis of SCD.