| 利用肌原纤维小片化指数推断死亡时间 |
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| 引用本文： | Wu RQ,Zhao ZQ,Shen YW,Jia JZ. 利用肌原纤维小片化指数推断死亡时间[J]. 法医学杂志, 2006, 22(1): 10-11,14 |
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| 作者姓名： | Wu RQ Zhao ZQ Shen YW Jia JZ |
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| 作者单位： | 复旦大学上海医学院法医系,上海,200032 |
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| 摘 要： | 目的研究死后机体骨骼肌肌原纤维小片化指数(MFI)与死亡时间之间的关系。方法双缩脲法测定室温下不同死后时间家兔骨骼肌全蛋白浓度,分光光度法测定540nm处0.5mg/mL蛋白浓度下的骨骼肌肌原纤维小片化指数。结果在死后早期,肌原纤维小片化指数随着时间延长逐步增高。结论肌原纤维小片化指数可用于早期死亡时间的推断。
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| 关 键 词： | 肌原纤维小片化指数(MFI) 死亡时间(PMI) 蛋白质降解 |
| 文章编号： | 1004-5619(2006)01-0010-02 |
| 修稿时间： | 2005-07-13 |
Estimation of postmortem interval using myofibril fragmentation index |
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| Wu Rong-qi,Zhao Zi-qin,Shen Yi-wen,Jia Jian-zhang. Estimation of postmortem interval using myofibril fragmentation index[J]. Journal of Forensic Medicine, 2006, 22(1): 10-11,14 |
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| Authors: | Wu Rong-qi Zhao Zi-qin Shen Yi-wen Jia Jian-zhang |
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| Affiliation: | Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032,China. wurongqi@gmail.com |
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| Abstract: | Objective To study the relationship between postmortem interval and the myofibril fragmentation index of skeletal muscle. Methods Rabbit skeletal muscle were left in the room temperature for different postmortem intervals, and the protein concentration of each sample was detected by using biuret method. Furthermore, the myofibril fragmentation index of each sample was measured under the protein concentration level of 0.5mg/mL. Results The myofibril fragmentation index increased obviously according to the postmortem interval prolongation. Conclusion The myofibril fragmentation index may be used on the estimation of early postmortem interval. |
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| Keywords: | myofibril fragmentation index(MFI) postmortem interval(PMI) protein degradation |
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