| 苯并二氮类药物及其主要代谢物的薄层色谱分析 |
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| 引用本文： | 吴玉红,谭家镒.苯并二氮类药物及其主要代谢物的薄层色谱分析[J].中国法医学杂志,2002,17(1). |
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| 作者姓名： | 吴玉红 谭家镒 |
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| 作者单位： | 中国刑警学院法医系,辽宁,沈阳,110035 |
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| 基金项目： | 国家重点科技“九五”攻关计划项目子专题“麻醉抢劫案中新毒物检测技术研究”基金资助 (No :96 9) |
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| 摘 要： | 目的建立饮料、血、尿中 11种常见苯并二氮类药物及尿中 7种主要代谢物的薄层色谱分析法(HPTLC)。方法分析物采用GDX10 1树脂进行固相萃取 ,乙醚作为洗脱溶剂。苯 :丙酮 (10∶6)等作为展开体系 ,改良碘化铋钾显色。结果所建方法绝对灵敏度 0 3～ 0 6μg,尿检出限 0 4～ 1 0 μg/ml、血检出限 0 6～ 1 0 μg/ml、饮料检出限 0 4～ 0 8μg/ml。结论HPTLC法简便、快速 ,适合作为常规毒物分析方法
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| 关 键 词： | 苯并二氮类药物 薄层色谱分析法(TLC) |
The analysis of benzodiazepines and its main metabolites by thin-layer chromatography |
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| WU Yuhong,TAN jiayi/.The analysis of benzodiazepines and its main metabolites by thin-layer chromatography[J].Chinese Journal of Forensic Medicine,2002,17(1). |
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| Authors: | WU Yuhong TAN jiayi/ |
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| Institution: | WU Yuhong,TAN jiayi/Department of Forensic Medicine,Criminal Police College of China,Shenyang 110035 |
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| Abstract: | Objective A method for the analysis of eleven benzodiazepine in beverages,blood and urine and seven main metabolites of benzodiazepine in urine by solid phase extraction and high performance thin layer chromatography is described in this paper.Method The analytic materials in specimens were extracted by using GDX 101 porous resin as absorbent and diethyl ether as eluant.The extracts were spread out on plates using benzene∶acetone(10∶6) as eluant and were visualized by modified Dragendorffs reagent.Results The absolute sensitivity of the analysis is 0 4 μg to 0 6 μg .The limit of detection of benzodiazepines in urine,blood and beverages are 0 4 μg/ml to 1 0 μg/ml ,0 6 μg/ml to 1 0 μg/ml and 0 4μg/ml to 0 8 μg/ml respectively.The limits of detection of metabolites of benzodiazepines in urine are 0 4 μg/ml to 1 0 μg/ml .Conclusion These results suggested that this method is useful for the detection of benzodiazepines in toxication case. |
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| Keywords: | benzodiazepines thin layer chromatography(TLC) |
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