MS31A位点MVR-PCR技术在法医学鉴定中的应用

应用小卫星特异性引物31A、31A -A、31A -G与α -32PdCTP掺入扩增技术对MS31A(位于D7S21位点)进行MVR -PCR分析 ,成功地建立了一套适合于刑事案件中微量血痕、混合斑、毛发、骨组织等样品鉴定的快速、简便、准确的分析技术。灵敏度分析表明 ,该方法能检测出低至1ng的基因组DNA ,具有较高的灵敏性。本技术在40余起强奸、凶杀等疑难案件中应用均获得成功。本研究还利用MVR数据库资料对实际案件中的犯罪嫌疑人成功地进行了筛选、排查以及串并案工作。

The application of minisatellite MS31A MVR-PCR digital coding technique in forensic science

Using isotope incorporate amplification technique of special 31A, 31A-A, 31A-G primers and alpha-32PdCTP, the minisatellite MS31A (located at D7S21 loci) was studied. A rapid, simple, and accurate MVR-PCR technique was successfully established. The technique can be applied in individual identification minutes amples, such as blood stains, semen stains contaminated by vaginal fluid, hair and bones. The sensitivity analysis revealed that this technique could detect 1 ng genoma DNA. It is also discribed about the application of the method in 40 criminal cases of rape and murder.