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PCR-RFLP分析线粒体DNA细胞色素b基因用于法医学种属鉴定
引用本文:冯强,陈浩,周斌,王晓娜,李楷,张博,张林. PCR-RFLP分析线粒体DNA细胞色素b基因用于法医学种属鉴定[J]. 刑事技术, 2006, 200(5): 16-18
作者姓名:冯强  陈浩  周斌  王晓娜  李楷  张博  张林
作者单位:1. 广东省深圳市南山公安分局,518052
2. 四川大学华西基础医学与法医学院,成都,610041
基金项目:四川省学术与技术带头人培养基金
摘    要:目的建立一种PCR-RFLP分析线粒体DNAcyt b基因用于法医学种属鉴定的方法。方法采用一对通用引物扩增人及15种常见动物的线粒体DNAcyt b基因,扩增产物经内切酶Alu I酶切,分析不同动物DNA扩增产物的有无以及酶切前后的变化。结果所检动物中有9种DNA有扩增产物,经内切酶Alu I酶切后,除鳝鱼外都能与人类DNA相区别。结论PCR-RFLP分析线粒体DNAcyt b基因方法简单,结果可靠,是一种较好的法医学种属鉴定方法。

关 键 词:法医学  种属鉴定
文章编号:1008-3650(2006)05-0016-03
修稿时间:2006-02-27

Species identification by PCR-RFLP analysis of the cytochrome b gene
FENG Qiang,CHEN Hao,ZHOU Bin,et al.. Species identification by PCR-RFLP analysis of the cytochrome b gene[J]. Forensic Science and Technology, 2006, 200(5): 16-18
Authors:FENG Qiang  CHEN Hao  ZHOU Bin  et al.
Abstract:Objective To establish a species identification method by PCR-RFLP analysis of the cytochrome b gene.Methods Using a pair of universal PCR primer,samples from 15 species of animals and human were amplified and the RFLP patterns were generated by the restriction endonuclease Alu I.Results The PCR-RFLP patterns of 15 species of animals except the mud eel were different from that of the human.Conclusion Identify the species by PCR-RFLP analysis of the cytochrome b gene is a simple and exact technique.It is valuable in species identification of forensic science.
Keywords:cyt b  PCR-RFLP
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