| PCR-RFLP分析线粒体DNA细胞色素b基因用于法医学种属鉴定 |
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| 引用本文： | 冯强,陈浩,周斌,王晓娜,李楷,张博,张林. PCR-RFLP分析线粒体DNA细胞色素b基因用于法医学种属鉴定[J]. 刑事技术, 2006, 200(5): 16-18 |
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| 作者姓名： | 冯强 陈浩 周斌 王晓娜 李楷 张博 张林 |
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| 作者单位： | 1. 广东省深圳市南山公安分局,518052
2. 四川大学华西基础医学与法医学院,成都,610041 |
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| 基金项目： | 四川省学术与技术带头人培养基金 |
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| 摘 要： | 目的建立一种PCR-RFLP分析线粒体DNAcyt b基因用于法医学种属鉴定的方法。方法采用一对通用引物扩增人及15种常见动物的线粒体DNAcyt b基因,扩增产物经内切酶Alu I酶切,分析不同动物DNA扩增产物的有无以及酶切前后的变化。结果所检动物中有9种DNA有扩增产物,经内切酶Alu I酶切后,除鳝鱼外都能与人类DNA相区别。结论PCR-RFLP分析线粒体DNAcyt b基因方法简单,结果可靠,是一种较好的法医学种属鉴定方法。
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| 关 键 词： | 法医学 种属鉴定 |
| 文章编号： | 1008-3650(2006)05-0016-03 |
| 修稿时间： | 2006-02-27 |
Species identification by PCR-RFLP analysis of the cytochrome b gene |
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| FENG Qiang,CHEN Hao,ZHOU Bin,et al.. Species identification by PCR-RFLP analysis of the cytochrome b gene[J]. Forensic Science and Technology, 2006, 200(5): 16-18 |
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| Authors: | FENG Qiang CHEN Hao ZHOU Bin et al. |
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| Abstract: | Objective To establish a species identification method by PCR-RFLP analysis of the cytochrome b gene.Methods Using a pair of universal PCR primer,samples from 15 species of animals and human were amplified and the RFLP patterns were generated by the restriction endonuclease Alu I.Results The PCR-RFLP patterns of 15 species of animals except the mud eel were different from that of the human.Conclusion Identify the species by PCR-RFLP analysis of the cytochrome b gene is a simple and exact technique.It is valuable in species identification of forensic science. |
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| Keywords: | cyt b PCR-RFLP |
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