Concordance Between the AmpFℓSTR® MiniFiler™ and AmpFℓSTR® Identifiler® PCR Amplification Kits in the Kuwaiti Population |
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| Authors: | Mohammad A. Alenizi,M.S., William Goodwin,Ph.D., Sibte Hadi,Ph.D., Homod H. Alenizi,B.S., Khaleda A. Altamar,B.S., Mona S. Alsikel,B.S. |
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| Affiliation: | School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, Lancashire, PR1 2HE, U.K.;
DNA Identification Laboratory, General Department of Criminal Evidence, State of Kuwait, Kuwait. |
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| Abstract: | Abstract: The AmpFℓSTR® MiniFiler™ polymerase chain reaction amplification kit, developed and supplied by Applied Biosystems, complements the AmpFℓSTR® Identifiler® polymerase chain reaction amplification kit (Applied Biosystems, Warrington, U.K.) by improving the success rate when profiling DNA that is degraded or contains inhibitors. Before applying the MiniFiler™ kit to casework, the profiles from 200 unrelated Kuwaitis were compared to Identifiler® profiles. Concordance was observed for 99.875% (1598 of 1600) of the compared STR loci. The two discordant profiles displayed allelic dropout: one at the D13S317 locus due to nonamplification of allele 10 in the MiniFiler™ profile, and one at the D18S51 locus due to nonamplification of allele 18 in the Identifiler® profile. |
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| Keywords: | forensic science DNA profiling short tandem repeats miniSTR concordance D13S317 D7S820 D2S1338 D21S11 D16S539 D18S51 CSF1PO FGA Amelogenin Kuwaiti population |
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