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DNA Preparation from Sexual Assault Cases by Selective Degradation of Contaminating DNA from the Victim |
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| Authors: | Alex M. Garvin,Ph.D., Michel Bottinelli,Ph.D., Mauro Gola,Ph.D., Ario Conti,Ph.D., Gianni Soldati,Ph.D. |
| Affiliation: | Bureco Corporation, Kagenstrasse 17, CH-4153 Reinach, Switzerland.; Molecular Diagnostic Laboratory, Division of Forensic Genetics, c/o Cardiocentro Ticino, Via Tesserete 48, CH-6900 Lugano, Switzerland.; Laboratory of Forensic Chemistry and Toxicology, Institute of Chemistry and Toxicology, 6718 Olivone, Switzerland. |
| Abstract: | Abstract: The standard method to purify sperm DNA from vaginal swabs taken from rape victims is to selectively digest the victim's epithelial cells to solubilize the victim's DNA, and then separate the soluble DNA from the intact sperm by centrifugation. A different approach to removing the soluble victim's DNA is to selectively degrade it using a nuclease, DNase I. DNase I reduces the amount of soluble DNA by over 1000-fold, while having virtually no effect on the sperm DNA remaining in the sperm head and inaccessible to the enzyme. Nuclease inactivation and sperm lysis then yield a soluble, pure male DNA fraction. An aliquot of soluble DNA is removed prior to nuclease addition to provide the victim's fraction. Vaginal swabs taken at defined time points following consensual sex and taken from rape victims were processed using the nuclease method or the standard method and the nuclease method gave superior short tandem repeat profiles. |
| Keywords: | forensic science sexual assault evidence automation selective degradation epithelial cells spermatozoa DNA typing PowerPlex 16 |