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| 高效液相色谱法测定小鼠血清中可卡因的含量 | |
| 引用本文： | 孙林,宋君,何威,任嘉谦. 高效液相色谱法测定小鼠血清中可卡因的含量[J]. 中国法医学杂志, 2002, 17(3): 165-166 |
| 作者姓名： | 孙林 宋君 何威 任嘉谦 |
| 作者单位： | 1. 中国医科大学实验技术中心,辽宁,沈阳,110001 2. 中国医科大学组胚教研室,辽宁,沈阳,110001 3. 中国医科大学脑研究所,辽宁,沈阳,110001 |
| 基金项目： | 国家自然科学基金资助,编号39970772 |
| 摘 要： | 目的 建立一种测定血清中可卡因含量的高效液相色谱方法。方法 以0.04mol/L磷酸盐缓冲液(含有0.26 mmol/L四丁基胺盐,pH3.2)-乙腈(83:17)为流动相,血清样品采用2％异戊醇正己烷-0.1 mol/L HCl液液反萃取法处理,用Kromasil C18色谱柱分析血清中可卡因的含量。紫外检测波长为235nm。结果可卡因的最小检出浓度为25ng/ml,回收率71％。结论所建方法测定血清中可卡因含量灵敏度高,简便易行。 |
| 关 键 词： | 毒物分析 可卡因 血清 HPLC 紫外检测 |
| 文章编号： | 1001-5728(2002)03-0165-02 |
| 修稿时间： | 2002-01-01 |
Determination of cocaine in rat''''s serum by high performance liquid chromatography |
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| SUN Lin,SONG Jun,HE Wei,et al.. Determination of cocaine in rat''''s serum by high performance liquid chromatography[J]. Chinese Journal of Forensic Medicine, 2002, 17(3): 165-166 | |
| Authors: | SUN Lin SONG Jun HE Wei et al. |
| Affiliation: | SUN Lin,SONG Jun,HE Wei,et al. Experiment Technology Centre,China Medical University,Shenyang 110001 |
| Abstract: | Objective To develop an assay method for quantitative analysis of cocaine in rat's serum by high-performance liquid chromatography (HPLC). Method Serum samples (30μl) were extracted with 2% isoamyl alcohol and hexane. The organic phase was extracted with 0.1 mol/L HC1. The quantitative analysis was achieved by using a 5μm reversed-phase Kromasil CIS column with mobile phase of 0.04mol/ L phosphate buffer solution (containing 0.26 mmol/L tetrabutylammonium chloride , pH3.2) - acetonitrile (83:17). The wave length of the ultraviolet detection was performed at 235nm. Results The lowest limit of the detection limit was 25ng/ml. The recovery of the cocaine was 71 %. Conclusion The method is sensitive, simple and easy to perform. |
| Keywords: | Toxicological analysis Cocaine Serum HPLC UV detection. |
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