| 用FLDAS-PCR和PCR-RFLP检测汉族人群GPT的多态性 |
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| 引用本文： | 赵彩霞,陈慧,宋海燕,黄代新,杨庆恩.用FLDAS-PCR和PCR-RFLP检测汉族人群GPT的多态性[J].中国法医学杂志,2003,18(2):90-92. |
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| 作者姓名： | 赵彩霞 陈慧 宋海燕 黄代新 杨庆恩 |
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| 作者单位： | 1. 青岛市中级人民法院技术室,山东,青岛,26607
2. 华中科技大学同济医学院法医学系,湖北,武汉,430030 |
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| 摘 要： | 目的 建立片段长度差异等位基因特异性PCR(FLDAS-PCR)检测GPT基因型的新方法,并调查武汉地区汉族人群GPT多态性分布。方法 应用FLDAS-PCR、PCR-RFLP分别对武汉地区248例汉族个体进行GPT基因型分型。结果 FLDAS-PCR与PCR-RFLP分型结果完全一致,GPT的3种基因型分型明确,基因频率GPT*1=0.5423,GPT*2=0.4577,基因型分布符合Hardy-Weinberg平衡。结论 FLDAS-PCR和PCR-RFSP分型GPT在法医学鉴定中有实用价值。
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| 关 键 词： | 法庭科学 谷丙转氨酶(GPT) 片段长度差异等位基因特异性PCR(FLDAS-PCR) 限制性片段长度多态性PCR-RFLP |
| 文章编号： | 1001-5728(2003)02-0090-03 |
| 修稿时间： | 2002年7月1日 |
A study on Genetic Polymorphism of GPT loci by FLDAS-PCR and PCR-RFLP |
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| ZHAO Caixia,CHEN Hui,SONG Haiyan,et al..A study on Genetic Polymorphism of GPT loci by FLDAS-PCR and PCR-RFLP[J].Chinese Journal of Forensic Medicine,2003,18(2):90-92. |
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| Authors: | ZHAO Caixia CHEN Hui SONG Haiyan |
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| Institution: | ZHAO Caixia,CHEN Hui,SONG Haiyan,et al. Qingdao Intermediate People's Court,Qingdao,Shandong,266071 |
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| Abstract: | Objective To establish a new allele specific PCR method for GPT typing: and study GPT polymorphism by FLDAS-PCR and PCR-RFLP. Method 248 unrelated individuals of Han were genotyped using FLDAS-PCR and PCR-RFLP. Results Three genotypes were identified with DP of 0.631 in Han population. Typing results by FLDAS-PCR were completely consistent with those by PCR-RFLP. The allele frequencies were GPT * 1 = 0.5423 and GPT * 2 = 0.4577. The population data fitted the Hardy-Weinberg law. Conclusion GPT-FLDAS-PCR and GPT-RFLP method is useful for forensic identification. |
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| Keywords: | Forensic science Glutamate pyruvate transaminase (GPT) Fragment length discrepant allele specific PCR (FLDAS-PCR) PCR restriction fragment length polymorphism (PCR-RFLP) |
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