| 用PCR技术鉴定自溶组织性别 |
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| 引用本文： | 卢英强,宣兆艳,张明,王洪波,郑永晨,王丽颖. 用PCR技术鉴定自溶组织性别[J]. 中国法医学杂志, 1994, 0(3) |
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| 作者姓名： | 卢英强 宣兆艳 张明 王洪波 郑永晨 王丽颖 |
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| 作者单位： | 白求恩医科大学法医教研室!长春，130021，白求恩医科大学法医教研室!长春，130021，白求恩医科大学法医教研室!长春，130021，白求恩医科大学法医教研室!长春，130021，白求恩医科大学第一临床医学院儿科!长春，130021，白求恩医科大学第一临床医学院儿科!长春，130021 |
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| 摘 要： | 本文用聚合酶链反应（PCR）检测已知男性各种不同程度自溶组织石蜡切片中的男性DNA序列。结果表明：自溶程度较低的组织可以用聚合酶链反应鉴定性别：但自溶程度达到核膜消失和细胞结构消失者，其性别鉴定阳性率明显降低，而且在自溶程度较高时，易出现假阴性结果。
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| 关 键 词： | 自溶组织 聚合酶链反应 性别鉴定 石蜡切片 |
SEX IDENTIFICATION OF AUTOLYSIS TISSUES BY POLYMERASE CHAIN REACTION |
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| Lu Yingqiang,Xuan Zhaoyan, Zhang Ming,Wang Hongbo. SEX IDENTIFICATION OF AUTOLYSIS TISSUES BY POLYMERASE CHAIN REACTION[J]. Chinese Journal of Forensic Medicine, 1994, 0(3) |
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| Authors: | Lu Yingqiang Xuan Zhaoyan Zhang Ming Wang Hongbo |
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| Abstract: | In this paper, the specific male sequeuce in paraffin section of various tissues with autolys is ofdifferent degrees was detected by polymerase chain reaction(PCR).The results shewed that PCR could be used for identifying sex in the tissues with low degrees ofautolysis; its positive rate was lower in high degrees with disappearance of nuclaic member and cell ou-tline,and often gave false negative results when the autolysis degree was high. |
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| Keywords: | Autolysis tissues Sex identification Polymerase chain reaction |
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