| Triton X-100快速提取甲醛固定、石蜡包埋组织内DNA的方法 |
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| 引用本文： | 贾怡,周斌,吕梅励,梁伟波,胡羽,张林. Triton X-100快速提取甲醛固定、石蜡包埋组织内DNA的方法[J]. 刑事技术, 2003, 0(6): 6-8,23 |
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| 作者姓名： | 贾怡 周斌 吕梅励 梁伟波 胡羽 张林 |
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| 作者单位： | 四川大学基础医学与法医学院,教育部人类疾病生物治疗重点实验室,成都,610041 |
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| 基金项目： | 国家自然科学基金(30171033)，教育部科学技术研究重点项目(01143)，教育部跨世纪优秀人才培养计划基金(2001-29) |
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| 摘 要： | 目的建立一种快速提取甲醛固定、石蜡包埋组织内DNA的方法。方法利用TritonX-100一步提取甲醛固定、石蜡包埋组织内DNA,并具体研究了不同浓度TritonX-100对提取后DNA-STR分型效果的影响。结果成功地一步提取出甲醛固定、石蜡包埋组织内DNA,浓度为1%的TritonX-100提取效果较好。结论利用TritonX-100提取甲醛固定、石蜡包埋组织内的DNA,为快速提取DNA提供了有效的途径,是法科学领域中一种值得推荐的方法。
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| 关 键 词： | TritonX-100 TH01 D8S1132 D7S820 法科学 |
| 文章编号： | 1008-3650(2003)06-0006-03 |
| 修稿时间： | 2003-01-03 |
A rapid method of DNA extraction from formalin-fixed paraffin-embedded tissues by using Triton X-100 |
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| JIA Yi,ZHOU Bin,LU Mei-li,et al. A rapid method of DNA extraction from formalin-fixed paraffin-embedded tissues by using Triton X-100[J]. Forensic Science and Technology, 2003, 0(6): 6-8,23 |
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| Authors: | JIA Yi ZHOU Bin LU Mei-li et al |
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| Abstract: | Objective Introducing a rapid method of DNA extract ion from formalin-fixed paraffin-embedded tissues.Methods The DNA of tissues from formalin-fixed paraffin-embedded are extracted by using Triton X-100 and the effect of DNA extract quantity in different concentration of Triton X-100solutions is analyzed.Result DNA are extracted successfully from formalin-fixed paraffin-embedded tissues and we find that a better DNA-STR typing re sult can be got by using1%Triton X-100solution.Conclusion It is determine d to be a valid method for rapidly extracting DNA from formalin-fixed paraffin-embedded tissues using Triton X-100and should be recommended. |
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| Keywords: | Triton X-100 TH01 D8S1132 D7S820 forensic science |
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