两组引物对无毛囊毛根的PCR-STR分型

目的了解无毛囊毛根的短扩增子引物和通用引物PCR-STR分型在法医实践中应用的可行性。方法应用5对Bulter设计的短扩增子引物D8S1179、D21S11、THO1、D16S539和VWA与其对应的STR通用引物,利用非变性PAGE,对无毛囊毛根进行PCR-STR分型的对比研究。结果短扩增子引物与通用引物对无毛囊毛根分型的正确率:D8S1179为11.4%和17.1%、D21S11为3.7%和5.9%、vWA为22.2%和33.3%、THO1为9.3%和5.6%、D16S539为0和9.2%。毛根的短扩增子引物和通用引物部分STR分型出现了干扰结果的Ladder-like背景条带。结论在对无毛囊毛根检材进行通用引物和短扩增子引物PCR-STR分型时,五个STR位点分型检测的成功率有限,还需注意Ladder-like条带的干扰。

A Comparison between two Primers of PCR-STR Typing for Hair Root without Sheath Cells

Objective To probe the feasibility of PCR-STR typing of hair root without sheath cells with current and redesigned miniSTR amplicon primers. Methods The DNA of the hair root without sheath cells is amplified by both current and miniSTR amplicon primers and checked by PAGE. Results The correct typing of hair root without sheath cells with MiniSTR amlicon primers and current primers is respectively: D8S1179 11.4%&17.1%;D21S11 3.7%&5.9%; vWA 22.2%&33.3%;THO1 9.3%&5.6%;D16S539 0&9.2%.The ladder-like bands detected in the PCR-STR typing of hair root without sheath cells is strongly disturb the result. Conclusion The DNA typing of hair root without sheath cells can be resolved limitedly with the two primers and Ladders-like bands should also be paid attention to in forensic casework.