| 用ELISA检测精液及精斑中精子抗原的研究 |
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| 引用本文： | 段亚清,徐晶华,王晓峰,张贯石,李玉新. 用ELISA检测精液及精斑中精子抗原的研究[J]. 中国法医学杂志, 1991, 0(4) |
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| 作者姓名： | 段亚清 徐晶华 王晓峰 张贯石 李玉新 |
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| 作者单位： | 白求恩医科大学法医教研室 长春130021 |
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| 摘 要： | 本实验应用单克隆抗人精子抗体和酶标记羊抗人精子抗体,采用ELISA方法确定精子抗原成份的存在。对10份新鲜精液,15份精斑进行了验测,其结果阳性率为100%。新鲜精液(精子数约10,000万个/ml)稀释100万倍,精斑浸出液稀释50万倍,均可出现阳性。对唾液斑、尿斑、乳汁斑、阴道斑、汗斑及输精管结扎的精液均为阴性。实验结果表明,本法检验精子抗原具有灵敏度高,特异性好的优点。
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| 关 键 词： | 单克隆抗人精子抗体 ELISA 精子抗原 精液 精斑 |
EXAMINATING THE SPERM ANTIGEN IN SEMINAL FLUID AND SEMINAL STAIN USING ELISA METHOD |
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| Duan Yaqing,Xu Jinghua,wang Xiaofeng,Zhang Guanshi,Li Yuxin. EXAMINATING THE SPERM ANTIGEN IN SEMINAL FLUID AND SEMINAL STAIN USING ELISA METHOD[J]. Chinese Journal of Forensic Medicine, 1991, 0(4) |
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| Authors: | Duan Yaqing Xu Jinghua wang Xiaofeng Zhang Guanshi Li Yuxin |
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| Affiliation: | Duan Yaqing;Xu Jinghua;wang Xiaofeng;Zhang Guanshi;Li Yuxin Department of forensic Medicine Norman Bethune University of Medical Sciences 130021 |
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| Abstract: | The sperm antigens were examined using the method of ELISA and specific monoclonal antiboby against human sperm.The results of ELISA method of 10 fresh seminal fluid samples and 15 seminal stains showed 100% positive rate. Even diluted the fresh seminal fluid to 10~6 times of seminal stains extract to 51×0~5 times,both were positive also.However,it was negtive for testing other specimens such as saliva stains,urine stains,colostrum stains,sweat stains, vaginal secretion stains and human seminal stains after vesoligation. The result certificate that using specific antibody to the sperm,the ELISA method is high sensitive and specific for the sperm antigen assay. |
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| Keywords: | Monoclonal antibody against human sperm ELISA sperm antigen Seminal fluid Seminal stain |
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