| 离子对SPE—HPLC法检测生物检材中的百草枯 |
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| 引用本文： | Wang RH,Su SM,Qin GM. 离子对SPE—HPLC法检测生物检材中的百草枯[J]. 法医学杂志, 2005, 21(2): 121-123 |
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| 作者姓名： | Wang RH Su SM Qin GM |
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| 作者单位： | 1. 昆明医学院法医学院,云南,昆明,650031
2. 玉溪市公安局刑科所,云南,玉溪,653100 |
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| 摘 要： | 目的建立生物检材中百草枯的简便、快速、灵敏、可靠的高效液相色谱分析方法。方法生物检材酶解后,用以十二烷基三甲基溴化铵和十二烷基硫酸钠预处理过的C18固相柱萃取,HPLC/DAD进行分析。结果回收率81%～94%,检出限为1ng·mL-1,线性范围50ng·mL-1～1mg·mL-1,结论此方法适用于中毒生物检材中百草枯的检测。
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| 关 键 词： | 百草枯 生物检材 固相萃取 |
| 文章编号： | 1004-5619(2005)02-0121-03 |
| 修稿时间： | 2004-12-07 |
Ion-pair solid-phase extraction (SPE) and HPLC analysis of paraquat in biological sample |
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| Wang Rui-hua,Su Shao-ming,Qin Guang-ming. Ion-pair solid-phase extraction (SPE) and HPLC analysis of paraquat in biological sample[J]. Journal of Forensic Medicine, 2005, 21(2): 121-123 |
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| Authors: | Wang Rui-hua Su Shao-ming Qin Guang-ming |
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| Affiliation: | Forensic Medicine School of Kunming Medical College, Kunming 650031, China. |
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| Abstract: | OBJECTIVE: To establish an HPLC method for the determination of Paraquat in biological samples. METHODS: Paraquat in biological samples was extracted by C18 columns which were pre-treated with cetyl-trimethyl ammonium bromide (CTAB) and soudium dodecyl sulphate (SDS), and analysed by HPLC/DAD. RESULTS: The detection limit of the method was 1 ng x mL(-1), and the average recoveries were 81%-94%. CONCLUSION: The method can be used to analysis of paraquat in biological samples. |
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| Keywords: | HPLC/DAD |
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