| 牙齿的DNA提取及STR分型研究 |
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| 引用本文： | 凃政,刘志芳,陈松,李万水. 牙齿的DNA提取及STR分型研究[J]. 刑事技术, 2007, 0(5): 9-11 |
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| 作者姓名： | 凃政 刘志芳 陈松 李万水 |
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| 作者单位： | 公安部物证鉴定中心,北京,100038 |
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| 摘 要： | 目的建立有效的牙齿DNA提取方法。方法使用物理及化学方法去除牙齿表面污染物,经脱钙、裂解、纯化从牙粉中提取DNA进行STR分型。结果对96例牙齿检材进行DNA检验,获得STR分型的有94例,在查找尸源的案件中发挥了重要作用。结论本方法操作简单快速,能够显著提高牙齿DNA检验的成功率。
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| 关 键 词： | 牙齿 DNA提取 复合扩增 STR分型 |
| 文章编号： | 1008-3650(2007)05-0009-03 |
| 修稿时间： | 2006-12-05 |
DNA extraction and STR genotyping from teeth |
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| TU Zheng,LIU Zhi-fang,CHEN Song,et al.. DNA extraction and STR genotyping from teeth[J]. Forensic Science and Technology, 2007, 0(5): 9-11 |
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| Authors: | TU Zheng LIU Zhi-fang CHEN Song et al. |
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| Abstract: | Objective To establish an efficient DNA extraction method for individual identification from teeth samples.Method The teeth DNA was extracted using cleansing,pulverizing,de-calcification,lysis and purification,followed by STR typing.Result 94 DNA profiles were successfully obtained from 96 teeth samples.Conclusion This new approach can increase the success rate of teeth DNA typing by streamlining extraction protocol. |
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| Keywords: | tooth DNA extraction multiplex amplification STR typing |
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